Gichuhi, J., Khamis, F. M., Van den Berg, J., Ekesi, S. and Herren, J. K. (2019) Unexpected diversity of Wolbachia associated with Bactrocera dorsalis (Diptera: Tephritidae) in Africa. Insects, 10(6), 155. (doi: 10.3390/insects10060155) (PMID:31159272) (PMCID:PMC6627279)
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Abstract
Bactrocera dorsalis (Hendel) is an important pest of fruit-bearing plants in many countries worldwide. In Africa, this pest has spread rapidly and has become widely established since the first invasion report in 2003. Wolbachia is a vertically transmitted endosymbiont that can significantly influence aspects of the biology and, in particular, the reproduction of its host. In this study, we screened B. dorsalis specimens collected from several locations in Africa between 2005 and 2017 for Wolbachia using a PCR-based assay to target the Wolbachia surface protein wsp. Of the 357 individuals tested, 10 were positive for Wolbachia using the wsp assay. We identified four strains of Wolbachia infecting two B. dorsalis mitochondrial haplotypes. We found no strict association between the infecting strain and host haplotype, with one strain being present in two different host haplotypes. All the detected strains belonged to Super Group B Wolbachia and did not match any strains reported previously in B. dorsalis in Asia. These findings indicate that diverse Wolbachia infections are present in invasive populations of B. dorsalis.
| Item Type: | Articles |
|---|---|
| Additional Information: | The authors gratefully acknowledge support for this research by the following organizations and agencies: the European Union Integrated Biological Control Applied Research Program (IBCARP)-Fruit Fly Component; the Wellcome trust [107372]; the UK’s Department for International Development (DFID); Swedish International Development Cooperation Agency (Sida); the Swiss Agency for Development and Cooperation (SDC); Federal Democratic Republic of Ethiopia and the Kenyan Government. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Herren, Dr Jeremy |
| Creator Roles: | |
| Authors: | Gichuhi, J., Khamis, F. M., Van den Berg, J., Ekesi, S., and Herren, J. K. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Insects |
| Publisher: | MDPI |
| ISSN: | 2075-4450 |
| ISSN (Online): | 2075-4450 |
| Published Online: | 31 May 2019 |
| Copyright Holders: | Copyright © 2019 The Authors |
| First Published: | First published in Insects 10(6): 155 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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