Intronic T-DNA insertion in Arabidopsis NBR1 conditionally affects wild-type transcript level

Collados Rodríguez, M. , Wawrzynska, A. and Sirko, A. (2014) Intronic T-DNA insertion in Arabidopsis NBR1 conditionally affects wild-type transcript level. Plant Signaling and Behavior, 9(12), e975659. (doi: 10.4161/15592324.2014.975659) (PMID:25482782) (PMCID:PMC4622546)

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Abstract

The SALK_135513 line of Arabidopsis thaliana is annotated by GenBank to have the T-DNA insertion in the fourth exon of NBR1 (At4g24690). Careful molecular analyses of the homozygous plants of SALK_135513 line indicated the place of T-DNA insertion in the fourth intron. Unexpectedly, 2 kinds of NBR1 transcripts, the wild-type and the mutated, resulting from alternative splicing events, were detected in those plants. Our findings explain the problems encountered by us with phenotypic evaluation of this line and emphasize the necessity for independent verification of the exact insertion site followed by careful expression studies when working with Arabidopsis T-DNA insertional mutants.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Collados Rodriguez, Dr Milagros
Authors: Collados Rodríguez, M., Wawrzynska, A., and Sirko, A.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
Journal Name:Plant Signaling and Behavior
Publisher:Taylor & Francis
ISSN:1559-2316
ISSN (Online):1559-2324
Published Online:31 October 2014
Copyright Holders:Copyright © 2014 Institute of Biochemistry and Biophysics, Polish Academy of Sciences
First Published:First published in Plant Signaling and Behavior 9(12): e975659
Publisher Policy:Reproduced under a Creative Commons License

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