Probing specificity of protein-protein interactions with chiral plasmonic nanostructures

Rodier, M., Keijzer, C., Milner, J. , Karimullah, A. , Barron, L., Gadegaard, N. , Lapthorn, A. and Kadodwala, M. (2019) Probing specificity of protein-protein interactions with chiral plasmonic nanostructures. Journal of Physical Chemistry Letters, 10, pp. 6105-6111. (doi: 10.1021/acs.jpclett.9b02288) (PMID:31549842)

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Protein–protein interactions (PPIs) play a pivotal role in many biological processes. Discriminating functionally important well-defined protein–protein complexes formed by specific interactions from random aggregates produced by nonspecific interactions is therefore a critical capability. While there are many techniques which enable rapid screening of binding affinities in PPIs, there is no generic spectroscopic phenomenon which provides rapid characterization of the structure of protein–protein complexes. In this study we show that chiral plasmonic fields probe the structural order and hence the level of PPI specificity in a model antibody–antigen system. Using surface-immobilized Fab′ fragments of polyclonal rabbit IgG antibodies with high specificity for bovine serum albumin (BSA), we show that chiral plasmonic fields can discriminate between a structurally anisotropic ensemble of BSA-Fab′ complexes and random ovalbumin (OVA)-Fab′ aggregates, demonstrating their potential as the basis of a useful proteomic technology for the initial rapid high-throughput screening of PPIs.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Lapthorn, Dr Adrian and Kadodwala, Professor Malcolm and Keijzer, Dr Chantal and Karimullah, Dr Affar and Barron, Professor Laurence and Milner, Dr Joel and Rodier, Ms Marion and Gadegaard, Professor Nikolaj
Authors: Rodier, M., Keijzer, C., Milner, J., Karimullah, A., Barron, L., Gadegaard, N., Lapthorn, A., and Kadodwala, M.
College/School:College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
College of Science and Engineering > School of Chemistry
College of Science and Engineering > School of Engineering
Journal Name:Journal of Physical Chemistry Letters
Publisher:American Chemical Society
ISSN (Online):1948-7185
Published Online:30 September 2019
Copyright Holders:Copyright © 2019 American Chemical Society
First Published:First published in Journal of Physical Chemistry Letters 10:6105-6111
Publisher Policy:Reproduced in accordance with the publisher copyright policy

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
173324Mapping the mesoscale structural landscape using "sculpted" chiral plasmonic fieldsMalcolm KadodwalaEngineering and Physical Sciences Research Council (EPSRC)EP/P00086X/1Chemistry