The receptor binding site of feline leukemia virus surface glycoprotein is distinct from the site involved in virus neutralization

Ramsey, I.K. , Spibey, N. and Jarrett, O. (1998) The receptor binding site of feline leukemia virus surface glycoprotein is distinct from the site involved in virus neutralization. Journal of Virology, 72(4), (PMID:9525654) (PMCID:PMC109800)

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The external surface glycoprotein (SU) of feline leukemia virus (FeLV) contains sites which define the viral subgroup and induce virus-neutralizing antibodies. The subgroup phenotypic determinants have been located to a small variable region, VR1, towards the amino terminus of SU. The sites which function as neutralizing epitopes in vivo are unknown. Recombinant SU proteins were produced by using baculoviruses that contained sequences encoding the SUs of FeLV subgroup A (FeLV-A), FeLV-C, and two chimeric FeLVs (FeLV-215 and FeLV-VC) in which the VR1 domain of FeLV-A had been replaced by the corresponding regions of FeLV-C isolates. The recombinant glycoproteins, designated Bgp70-A, -C, -215, and -VC, respectively, were similar to their wild-type counterparts in several immunoblots and inhibited infection of susceptible cell lines in a subgroup-specific manner. Thus, Bgp70-A interfered with infection by FeLV-A, whereas Bgp70-C, -VC, and -215 did not. Conversely, Bgp70-C, -VC, and -215 blocked infection with FeLV-C, while Bgp70-A had no effect. These results indicate that the site on SU which binds to the FeLV cell surface receptor was preserved in the recombinant glycoproteins. It was also found that the recombinant proteins were able to bind naturally occurring neutralizing antibodies. Bgp70-A, -VC, and -215 interfered with the action of anti-FeLV-A neutralizing antibodies, whereas Bgp70-C did not. Furthermore, Bgp70-C interfered with the action of anti-FeLV-C neutralizing antibodies, while the other proteins did not. These results indicate that the neutralizing epitope(s) of FeLV SU lies outside the subgroup-determining VR1 domain.

Item Type:Articles
Additional Information:I.K.R. received a Wellcome Trust Veterinary Research Training Scholarship during this project. N.S. was supported by Intervet International.
Glasgow Author(s) Enlighten ID:Jarrett, Professor James and Ramsey, Professor Ian
Authors: Ramsey, I.K., Spibey, N., and Jarrett, O.
College/School:College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
Journal Name:Journal of Virology
Publisher:American Society for Microbiology
ISSN (Online):1098-5514

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