A novel genus in the order Picornavirales detected in human stool

Oude Munnink, B. B., Cotten, M. , Bakker, M., van der Hoek, L., Jebbink, M. F., Kellam, P., Jazaeri Farsani, S. M., Deijs, M. and Canuti, M. (2015) A novel genus in the order Picornavirales detected in human stool. Journal of General Virology, 96(11), pp. 3440-3443. (doi: 10.1099/jgv.0.000279) (PMID:26354795)

Abstract

Recently, four new viruses belonging to an unassigned family within the order Picornavirales were identified in excrements of healthy carp (fisavirus) and pigs (posavirus 1, 2 and 3). We report the detection and characterization of a fifth virus present in human faeces. The virus, named human stool-associated RNA virus (husavirus), contains a single ORF encoding a putative 2993 AA polyprotein, with a Hel-Pro-Pol replication block, typical for the Picornavirales. Phylogenetic analysis revealed that the closest relative to husavirus is posavirus 1, and together they cluster with fisavirus, posavirus 2 and 3 and a roundworm (Ascaris suum) derived virus. Husavirus was detected in eight human stool samples collected in 1984 (n = 2), 1985 (n = 4), 1995 (n = 1) and 2014 (n = 1). From three strains of husavirus from 1984 and 1985 the full genome sequence was determined, showing less than 5 % intraspecies variation in the nucleotide composition. The host of this virus remains to be determined.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Cotten, Professor Matthew
• Authors: Oude Munnink, B. B., Cotten, M., Bakker, M., van der Hoek, L., Jebbink, M. F., Kellam, P., Jazaeri Farsani, S. M., Deijs, M., and Canuti, M.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity; College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
• Journal Name: Journal of General Virology
• Publisher: Microbiology Society
• ISSN: 0022-1317
• ISSN (Online): 1465-2099
• Published Online: 01 November 2015