Chung, L. H. C., Birch, D. J. S., Vyshemirsky, V. , Ryadnov, M. G. and Rolinski, O. J. (2019) Tracking insulin glycation in real time by time-resolved emission spectroscopy. Journal of Physical Chemistry B, 123(37), pp. 7812-7817. (doi: 10.1021/acs.jpcb.9b06363) (PMID:31441653)
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Abstract
The application of time-resolved fluorescence sensing to the study of heterogenic biomolecular systems remains challenging because of the complexity of the resulting photophysics. Measuring the time-resolved emission spectroscopy (TRES) spectra can provide a more informative alternative to the modeling of the fluorescence decay that is currently employed. Here, we demonstrate this approach by monitoring real-time changes in intrinsic insulin fluorescence by TRES as a straightforward probe to directly measure kinetics of insulin aggregation and glycation. Our findings hold promise for monitoring the storage of insulin and its application in the control of diabetes and may support the development of more effective therapeutics against amyloidosis.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Vyshemirsky, Dr Vladislav |
Authors: | Chung, L. H. C., Birch, D. J. S., Vyshemirsky, V., Ryadnov, M. G., and Rolinski, O. J. |
College/School: | College of Science and Engineering > School of Mathematics and Statistics > Statistics |
Journal Name: | Journal of Physical Chemistry B |
Publisher: | American Chemical Society |
ISSN: | 1520-6106 |
ISSN (Online): | 1520-5207 |
Published Online: | 23 August 2019 |
Copyright Holders: | Copyright © 2019 American Chemical Society |
First Published: | First published in Journal of Physical Chemistry B 123(37): 7812-7817 |
Publisher Policy: | Reproduced in accordance with the publisher copyright policy |
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