Tracking insulin glycation in real time by time-resolved emission spectroscopy

Chung, L. H. C., Birch, D. J. S., Vyshemirsky, V. , Ryadnov, M. G. and Rolinski, O. J. (2019) Tracking insulin glycation in real time by time-resolved emission spectroscopy. Journal of Physical Chemistry B, 123(37), pp. 7812-7817. (doi: 10.1021/acs.jpcb.9b06363) (PMID:31441653)

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Abstract

The application of time-resolved fluorescence sensing to the study of heterogenic biomolecular systems remains challenging because of the complexity of the resulting photophysics. Measuring the time-resolved emission spectroscopy (TRES) spectra can provide a more informative alternative to the modeling of the fluorescence decay that is currently employed. Here, we demonstrate this approach by monitoring real-time changes in intrinsic insulin fluorescence by TRES as a straightforward probe to directly measure kinetics of insulin aggregation and glycation. Our findings hold promise for monitoring the storage of insulin and its application in the control of diabetes and may support the development of more effective therapeutics against amyloidosis.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Vyshemirsky, Dr Vladislav
Authors: Chung, L. H. C., Birch, D. J. S., Vyshemirsky, V., Ryadnov, M. G., and Rolinski, O. J.
College/School:College of Science and Engineering > School of Mathematics and Statistics > Statistics
Journal Name:Journal of Physical Chemistry B
Publisher:American Chemical Society
ISSN:1520-6106
ISSN (Online):1520-5207
Published Online:23 August 2019
Copyright Holders:Copyright © 2019 American Chemical Society
First Published:First published in Journal of Physical Chemistry B 123(37): 7812-7817
Publisher Policy:Reproduced in accordance with the publisher copyright policy

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