Computational image analysis of guided acoustic waves enables rheological assessment of sub-nanoliter volumes

Khalid, M. A. , Ray, A., Cohen, S., Tassieri, M. , Demčenko, A. , Tseng, D., Reboud, J. , Ozcan, A. and Cooper, J. M. (2019) Computational image analysis of guided acoustic waves enables rheological assessment of sub-nanoliter volumes. ACS Nano, 13(10), pp. 11062-11069. (doi: 10.1021/acsnano.9b03219) (PMID:31490647)

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We present a method for the computational image analysis of high frequency guided sound waves based upon the measurement of optical interference fringes, produced at the air interface of a thin film of liquid. These acoustic actuations induce affine deformations in the liquid, creating a lensing effect that can be readily observed using a simple imaging system. We exploit this effect to measure and analyze the spatio-temporal behavior as the acoustic wave interacts with the liquid. We also show that, by measuring the dynamics of the relaxation processes of these deformations when actuation ceases, we are able to determine the liquid's viscosity using just a lens-free optical sensor and a simple disposable biochip. Contrary to all other acoustic-based techniques in rheology, our measurements do not require monitoring of the wave parameters to obtain quantitative values for fluid viscosities, for sample volumes as low as 200 pL. We envisage that the proposed methods could enable high throughput, chip-based, reagent-free rheological studies within very small samples.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Demcenko, Dr Andriejus and Tassieri, Dr Manlio and Khalid, Mr Arslan and Cooper, Professor Jonathan and Reboud, Professor Julien
Authors: Khalid, M. A., Ray, A., Cohen, S., Tassieri, M., Demčenko, A., Tseng, D., Reboud, J., Ozcan, A., and Cooper, J. M.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:ACS Nano
Publisher:American Chemical Society
ISSN (Online):1936-086X
Published Online:06 September 2019
Copyright Holders:Copyright © 2019 American Chemical Society
First Published:First published in ACS Nano 13(10):11062-11069
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
617021Advanced Diagnostics using PhononicsJonathan CooperEngineering and Physical Sciences Research Council (EPSRC)EP/K027611/1ENG - BIOMEDICAL ENGINEERING
625501Bio-PHONONICS: Advanced Microfluidics & Diagnostics using Acoustic Holograms Bio-PhononicsJonathan CooperEuropean Research Council (ERC)340117ENG - BIOMEDICAL ENGINEERING
3014410Experiencing the micro-world - a cell's perspectiveManlio TassieriEngineering and Physical Sciences Research Council (EPSRC)EP/R035067/1ENG - Biomedical Engineering