Effect of peroxynitrite (ONOO−) on the function of murine perivascular adipose tissue

Ugusman, A. B., Riddell, A. and Kennedy, S. (2019) Effect of peroxynitrite (ONOO−) on the function of murine perivascular adipose tissue. Pharmacology and Pharmacy, 10(4), pp. 194-204. (doi: 10.4236/pp.2019.104017)

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Perivascular adipose tissue (PVAT) surrounds the exterior of blood vessels and releases numerous substances such as adiponectin which positively modulate blood vessel tone. In some cardiovascular diseases such as diabetes and high blood pressure, the function of PVAT changes and we speculated that oxidant stress may play a role in this change. PVAT has the ability to generate both superoxide and nitric oxide and these can combine rapidly under physiological conditions to form peroxynitrite (ONOO-). In disease states, the production of ONOO- may be increased and so its effect on the function of PVAT is of great interest. Consequently, we studied the effects of acute addition of the oxidant species ONOO- on vascular tone and production of adiponectin by mouse thoracic aortic PVAT. Murine PVAT immunostained for nitrotyrosine, indicating that ONOO- is formed in the PVAT. Exogenous ONOO- significantly increased the anticontractile effect of PVAT via increased adiponectin content but had no effect on eNOS expression or phosphorylation. These results suggest that generation of ONOO- within PVAT may be an important regulatory mechanism which influences the activity of PVAT. The effect of chronic exposure to raised levels of ONOO- on PVAT function remains to be determined.

Item Type:Articles
Additional Information:Azizah Binti Ugusman was supported by a visiting scholarship from the National University of Malaysia and Ministry of Higher. Alex Riddell was supported by an MRes/PhD studentship from the British Heart Foundation.
Glasgow Author(s) Enlighten ID:Riddell, Dr Alexandra and Kennedy, Professor Simon
Authors: Ugusman, A. B., Riddell, A., and Kennedy, S.
Subjects:R Medicine > RM Therapeutics. Pharmacology
College/School:College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health
College of Medical Veterinary and Life Sciences > School of Life Sciences
Journal Name:Pharmacology and Pharmacy
Publisher:Scientific Publishing
ISSN (Online):2157-9431
Published Online:23 April 2019
Copyright Holders:Copyright © 2019 The Authors
First Published:First published in Pharmacology and Pharmacy 10(4): 194-204
Publisher Policy:Reproduced under a Creative Commons License

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