2'-O-methyl, 2'-O-ethyl oligoribonucleotides and phosphorothioate oligodeoxyribonucleotides as inhibitors of the in vitro U7 snRNP-dependent mRNA processing event

Cotten, M. , Oberhauser, B., Brunar, H., Holzner, A., Issakides, G., Noe, C. R., Schaffner, G., Wagner, E. and Brinstiel, M. L. (1991) 2'-O-methyl, 2'-O-ethyl oligoribonucleotides and phosphorothioate oligodeoxyribonucleotides as inhibitors of the in vitro U7 snRNP-dependent mRNA processing event. Nucleic Acids Research, 19(10), pp. 2629-2635. (doi: 10.1093/nar/19.10.2629) (PMID:1710340) (PMCID:PMC328180)

Abstract

We describe the synthesis of 2′-O-methyl, 2′-O-ethyl oligoribonucleotides and phosphorothioate oligodeoxyribonucleotides and demonstrate their utility as Inhibitors of the in vitro U7 snRNP-dependent mRNA processing event. These 2′-O-modifled compounds were designed to possess the binding affinity of an RNA molecule towards a complementary RNA target with an enhanced stability against nucleases. The 2′-O-methyl and 2′-O-ethyl antisense compounds function as potent inhibitors of the reaction at 1–10 nM, approximately 5-fold more effective than a natural antisense RNA molecule and requiring an approximate 5-fold excess over the target RNA for 80% inhibition of the processing reaction.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Cotten, Professor Matthew
• Authors: Cotten, M., Oberhauser, B., Brunar, H., Holzner, A., Issakides, G., Noe, C. R., Schaffner, G., Wagner, E., and Brinstiel, M. L.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity; College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
• Journal Name: Nucleic Acids Research
• Publisher: Oxford University Press
• ISSN: 1362-4962
• ISSN (Online): 1362-4962