Amphibian oocytes and sphere organelles: are the U snRNA genes amplified?

Phillips, S., Cotten, M. , Laengle-Rouault, F., Schaffner, G. and Birnstiel, M. L. (1992) Amphibian oocytes and sphere organelles: are the U snRNA genes amplified? Chromosoma, 101(9), pp. 549-556. (doi:10.1007/bf00660314) (PMID:1521499)

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Abstract

The sphere organelles (spheres) ofXenopus and other amphibian oocytes are known to contain small nuclear ribonucleoprotein particles (snRNPs) and have been suggested to play a role in snRNP complex assembly. Coupled with the similarities that exist between spheres and nucleoli and the quantitative and kinetic aspects of snRNA synthesis in theXenopus oocyte, we have investigated whether or not the U snRNA encoding genes are amplified inXenopus oogenesis, the spheres being possible sites for the location of such extrachromosomal gene copies. By applying a number of quantitative nucleic acid hybridization procedures to both total and fractionated oocyte and somatic DNA, employing both homologous and heterologous U snRNA gene probes and suitable amplification and non-amplification control probes, we show that the U snRNA genes do not undergo any major amplification inXenopus oogenesis. Therefore, the analogy between the sphere organelles and nucleoli appears to be limited. The role of the spheres and their relationship to other snRNP containing structures, specifically B snurposomes, and the sphere organizer loci remains obscure.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Cotten, Professor Matthew and Phillips, Professor Stephen
Authors: Phillips, S., Cotten, M., Laengle-Rouault, F., Schaffner, G., and Birnstiel, M. L.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Chromosoma
Publisher:Springer
ISSN:0009-5915
ISSN (Online):1432-0886

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