Zatloukal, K., Schneeberger, A., Berger, M., Koszik, F., Schmidt, W., Wagner, E., Cotten, M. , Buschle, M., Maass, G. and Stingl, G. (1994) Genetic modification of cells by receptor-mediated adenovirus-augmented gene delivery: a new approach for immunotherapy of cancer. Verhandlungen der Deutschen Gesellschaft fur Pathologie, 78, pp. 171-176. (PMID:7533981)
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Abstract
Most concepts of gene therapy of cancer are based on the generation of an enhanced immune response against the cancer by means of vaccination with gene-modified cancer cells. We have investigated the applicability of a new gene transfer technique which uses the receptor-mediated endocytosis pathway and the endosome disruption activity of adenovirus for the generation of a cancer vaccine consisting of interleukin-2 (IL-2)-transfected, irradiated murine melanoma cells (clone M-3). This technique resulted in very high IL-2 expression (in the range of 30,000 Units IL-2/10(6) cells/24 hrs) in the transfected cells without the need to selection of stably expressing cell clones. We found that this high IL-2 expression of the melanoma cells correlates with high efficacy of the vaccine. Immunization of animals with this vaccine elicits a systemic T-cell-mediated immune response which protects from tumor development after implantation of highly tumorigenic doses of wild-type melanoma cells.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Cotten, Professor Matthew |
| Authors: | Zatloukal, K., Schneeberger, A., Berger, M., Koszik, F., Schmidt, W., Wagner, E., Cotten, M., Buschle, M., Maass, G., and Stingl, G. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
| Journal Name: | Verhandlungen der Deutschen Gesellschaft fur Pathologie |
| Publisher: | Deutsche Gesellschaft fuer Pathologie e.V. |
| ISSN: | 0070-4113 |
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