Expression of coiled-coil protein 1, a novel gene downstream of FGF2, in the developing brain

Pellicano, F., Inglis-Broadgate, S. L., Pante, G., Ansorge, W. and Iwata, T. (2006) Expression of coiled-coil protein 1, a novel gene downstream of FGF2, in the developing brain. Gene Expression Patterns, 6(3), pp. 285-293. (doi: 10.1016/j.modgep.2005.07.004)

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Abstract

Fibroblast growth factor 2 (FGF2) plays an important role in cortical development. However, the genes downstream of FGF2 that mediate its effect are largely unknown. We have performed a microarray screening of genes regulated by FGF2 using primary cortical neuron culture derived from embryonic day 14.5 (E14.5) mouse forebrains. In this study, we have analysed a previously uncharacterised gene encoding a 180-amino acid protein, hereby named ‘coiled-coil protein 1 (ccp1)’, that showed a modest up-regulation upon FGF2 stimulation. Northern blots and RT-PCR showed specific expression of ccp1 in multiple tissues including adult and embryonic brains. In situ hybridizations revealed that ccp1 was expressed in the cortical plate between Reelin and Tbr1-positive layers in the dorsal cortex at E15.5. Furthermore, the expression pattern of ccp1 at E13.5–E14.5 reflected some of the aspects of tangential migration of cortical progenitors during the early phase. We observed that the expressed ccp1 protein was localised to endo/lysosomal compartment in the cell body as well as to vesicles present in the processes of primary cortical neurons and oligodendrocyte cell line.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Pellicano, Dr Francesca and Iwata, Dr Tomoko
Authors: Pellicano, F., Inglis-Broadgate, S. L., Pante, G., Ansorge, W., and Iwata, T.
College/School:College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Clinical Specialities
College of Medical Veterinary and Life Sciences > School of Cancer Sciences
Journal Name:Gene Expression Patterns
ISSN:1567-133X
ISSN (Online):1872-7298
Published Online:27 December 2005

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