Semi-intact ex vivo approach to investigate spinal somatosensory circuits

Hachisuka, J. , Baumbauer, K. M., Omori, Y., Snyder, L. M., Koerber, H. R. and Ross, S. E. (2016) Semi-intact ex vivo approach to investigate spinal somatosensory circuits. eLife, 5, e22866. (doi: 10.7554/eLife.22866) (PMID:27991851) (PMCID:PMC5214752)

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Abstract

The somatosensory input that gives rise to the perceptions of pain, itch, cold and heat are initially integrated in the superficial dorsal horn of the spinal cord. Here, we describe a new approach to investigate these neural circuits in mouse. This semi-intact somatosensory preparation enables recording from spinal output neurons, while precisely controlling somatosensory input, and simultaneously manipulating specific populations of spinal interneurons. Our findings suggest that spinal interneurons show distinct temporal and spatial tuning properties. We also show that modality selectivity — mechanical, heat and cold — can be assessed in both retrogradely labeled spinoparabrachial projection neurons and genetically labeled spinal interneurons. Finally, we demonstrate that interneuron connectivity can be determined via optogenetic activation of specific interneuron subtypes. This new approach may facilitate key conceptual advances in our understanding of the spinal somatosensory circuits in health and disease.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Hachisuka, Dr Junichi
Authors: Hachisuka, J., Baumbauer, K. M., Omori, Y., Snyder, L. M., Koerber, H. R., and Ross, S. E.
College/School:College of Medical Veterinary and Life Sciences > Institute of Neuroscience and Psychology
Journal Name:eLife
Publisher:eLife Sciences Publications
ISSN:2050-084X
ISSN (Online):2050-084X
Copyright Holders:Copyright © 2016 Hachisuka et al.
First Published:First published in eLife 5: e22866
Publisher Policy:Reproduced under a Creative Commons License

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