Jerome, H. et al. (2019) Metagenomic next-generation sequencing aids the diagnosis of viral infections in febrile returning travellers. Journal of Infection, 79(4), pp. 383-388. (doi: 10.1016/j.jinf.2019.08.003) (PMID:31398374) (PMCID:PMC6859916)
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Abstract
Objectives: Travel-associated infections are challenging to diagnose because of the broad spectrum of potential aetiologies. As a proof-of-principle study, we used MNGS to identify viral pathogens in clinical samples from returning travellers in a single center to explore its suitability as a diagnostic tool. Methods: Plasma samples from 40 returning travellers presenting with a fever of ≥38°C were sequenced using MNGS on the Illumina MiSeq platform and compared with standard-of-care diagnostic assays. Results: In total, 11/40 patients were diagnosed with a viral infection. Standard of care diagnostics revealed 5 viral infections using plasma samples; dengue virus 1 (n = 2), hepatitis E (n = 1), Ebola virus (n = 1) and hepatitis A (n = 1), all of which were detected by MNGS. Three additional patients with Chikungunya virus (n = 2) and mumps virus were diagnosed by MNGS only. Respiratory infections detected by nasal/throat swabs only were not detected by MNGS of plasma. One patient had infection with malaria and mumps virus during the same admission. Conclusions: MNGS analysis of plasma samples improves the sensitivity of diagnosis of viral infections and has potential as an all-in-one diagnostic test. It can be used to identify infections that have not been considered by the treating physician, co-infections and new or emerging pathogens. Summary: Next generation sequencing (NGS) has potential as an all-in-one diagnostic test. In this study we used NGS to diagnose returning travellers with acute febrile illness in the UK, highlighting cases where the diagnosis was missed using standard methods.
| Item Type: | Articles |
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| Keywords: | Diagnosis, NGS, returning travellers. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Harder, Miss Lisbeth and Davis, Dr Chris and Jesudason, Dr Natasha and Gunson, Dr Rory and Da Silva Filipe, Dr Ana and Vattipally, Dr Sreenu and Thomson, Professor Emma and Wilson-Davies, Dr Eleri and Ashraf, Dr Shirin |
| Authors: | Jerome, H., Taylor, C., Sreenu, V. B., Klymenko, T., Da Silva Filipe, A., Jackson, C., Davis, C., Ashraf, S., Wilson-Davies, E., Jesudason, N., Devine, K., Harder, L., Aitken, C., Gunson, R., and Thomson, E. C. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
| Journal Name: | Journal of Infection |
| Publisher: | Elsevier |
| ISSN: | 0163-4453 |
| ISSN (Online): | 1532-2742 |
| Published Online: | 06 August 2019 |
| Copyright Holders: | Copyright © 2019 The Authors |
| First Published: | First published in Journal of Infection 79(4): 383-388 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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