Connolly, J. P.R., O'Boyle, N., Turner, N., Browning, D. F. and Roe, A. J. (2019) Distinct intra-species virulence mechanisms regulated by a conserved transcription factor. Proceedings of the National Academy of Sciences of the United States of America, 116(39), pp. 19695-19704. (doi: 10.1073/pnas.1903461116) (PMID:31501343) (PMCID:PMC6765310)
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Abstract
Bacterial pathogens emerge by adapting mechanisms of virulence, differentiating them from their nonpathogenic progenitor. Virulence factors are often encoded on accessory genomic elements not part of the core genome and therefore must be integrated into the regulatory architecture of the cell. Here, we show that a highly conserved transcription factor in Escherichia coli has been relieved of a common purpose and adapted to regulate virulence pleiotropically in 2 distinct genetic backgrounds. This leads to enhanced virulence of both intestinal enterohemorrhagic E. coli and extraintestinal uropathogenic E. coli by exclusive mechanisms. These findings challenge the assumption that conserved transcription factors regulate common pathways maintained within a species and suggest that transcriptional repurposing creates new primary roles on an individual basis.
| Item Type: | Articles |
|---|---|
| Additional Information: | This work was supported by Biotechnology and Biological Sciences Research Council (BBSRC) funding (BB/M029646/1 and BB/ R006539/1) awarded to A.J.R., and a Wellcome Trust Institutional Strategic Support Fund (204820/Z/16/Z) awarded to J.P.R.C. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Turner, Natasha and Connolly, Dr James and Roe, Professor Andrew and O Boyle, Dr Nicky |
| Authors: | Connolly, J. P.R., O'Boyle, N., Turner, N., Browning, D. F., and Roe, A. J. |
| College/School: | College of Medical Veterinary and Life Sciences College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Proceedings of the National Academy of Sciences of the United States of America |
| Publisher: | National Academy of Sciences |
| ISSN: | 0027-8424 |
| ISSN (Online): | 1091-6490 |
| Published Online: | 09 September 2019 |
| Copyright Holders: | Copyright © 2019 The Authors |
| First Published: | First published in Proceedings of the National Academy of Sciences of the United States of America 116(39): 19695-19704 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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