Furnon, W. et al. (2019) Remodeling of the actin network associated with the non-structural protein 1 (NS1) of West Nile virus and formation of NS1-containing tunneling nanotubes. Viruses, 11(10), 901. (doi: 10.3390/v11100901) (PMID:31569658)
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191710.pdf - Published Version Available under License Creative Commons Attribution. 6MB |
Abstract
The cellular response to the recombinant NS1 protein of West Nile virus (NS1WNV) was studied using three different cell types: Vero E6 simian epithelial cells, SH-SY5Y human neuroblastoma cells, and U-87MG human astrocytoma cells. Cells were exposed to two different forms of NS1WNV: (i) the exogenous secreted form, sNS1WNV, added to the extracellular milieu; and (ii) the endogenous NS1WNV, the intracellular form expressed in plasmid-transfected cells. The cell attachment and uptake of sNS1WNV varied with the cell type and were only detectable in Vero E6 and SH-SY5Y cells. Addition of sNS1WNV to the cell culture medium resulted in significant remodeling of the actin filament network in Vero E6 cells. This effect was not observed in SH-SY5Y and U-87MG cells, implying that the cellular uptake of sNS1WNV and actin network remodeling were dependent on cell type. In the three cell types, NS1WNV-expressing cells formed filamentous projections reminiscent of tunneling nanotubes (TNTs). These TNT-like projections were found to contain actin and NS1WNV proteins. Interestingly, similar actin-rich, TNT-like filaments containing NS1WNV and the viral envelope glycoprotein EWNV were also observed in WNV-infected Vero E6 cells.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Furnon, Dr Wilhelm and Ratinier, Dr Maxime and Arnaud, Mr Frederick |
| Creator Roles: | Furnon, W.Methodology, Formal analysis, Investigation Ratinier, M.Methodology, Formal analysis Arnaud, F.Methodology, Formal analysis |
| Authors: | Furnon, W., Fender, P., Confort, M.-P., Desloire, S., Nangola, S., Kitidee, K., Leroux, C., Ratinier, M., Arnaud, F., Lecollinet, S., Boulanger, P., and Hong, S. S. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
| Journal Name: | Viruses |
| Publisher: | MDPI |
| ISSN: | 1999-4915 |
| ISSN (Online): | 1999-4915 |
| Published Online: | 27 September 2019 |
| Copyright Holders: | Copyright © 2019 The Authors |
| First Published: | First published in Viruses 11(10): 901 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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