Rezig, I. M., Bremner, S. K., Bhutta, M. S., Salt, I. P. , Gould, G. W. and McInerny, C. J. (2019) Genetic and cytological methods to study ESCRT cell cycle function in fission yeast. In: Culetto, E. and Legouis, R. (eds.) The ESCRT Complexes: Methods and Protocols. Series: Methods in molecular biology (1998). Humana Press: New York, pp. 239-250. ISBN 9781493994915 (doi: 10.1007/978-1-4939-9492-2_18)
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Abstract
The fission yeast Schizosaccharomyces pombe, an ascomycete fungus, is an established model organism for studying eukaryotic molecular and cellular events such as the cell cycle due to its powerful genetics, a sequenced genome, and the ease of molecular manipulation (Wood et al., Nature 415:871-880, 2002; Hoffman et al., Genetics 201:403-423, 2015). This chapter describes genetic and cytological methods to study endosomal sorting complex required for transport (ESCRT) function during the cell cycle in fission yeast. These include tetrad analysis to allow the creation of double mutants to test for genetic interactions by synthetic phenotype characterization, such as cellular growth and the analysis of division septa by calcofluor-white staining.
| Item Type: | Book Sections |
|---|---|
| Keywords: | Anillin, calcofluor-white, ESCRT, fission yeast, genetic interaction, polo kinase, Schizosaccharomyces pombe, septation, synthetic phenotype, tetrad analysis. |
| Status: | Published |
| Glasgow Author(s) Enlighten ID: | rezig, Imane Nour El Houda and BREMNER, SHAUN and McInerny, Dr Chris and Gould, Professor Gwyn and Salt, Dr Ian |
| Authors: | Rezig, I. M., Bremner, S. K., Bhutta, M. S., Salt, I. P., Gould, G. W., and McInerny, C. J. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
| Journal Name: | Methods in molecular biology (Clifton, N.J.) |
| Publisher: | Humana Press |
| ISSN: | 1940-6029 |
| ISBN: | 9781493994915 |
| Published Online: | 28 June 2019 |
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