Bamford, C., Wignall-Fleming, E., Sreenu, V. B. , Randall, R., Duprex, P. and Rima, B. (2019) Unusual, stable replicating viruses generated from mumps virus cDNA clones. PLoS ONE, 14(7), e0219168. (doi: 10.1371/journal.pone.0219168) (PMID:31276568)
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Abstract
In reverse genetic experiments we have isolated recombinant mumps viruses (rMuV) that carry large numbers of mutations clustered in small parts of their genome, which are not caused by biased hyper-mutation. In two separate experiments we obtained such recombinant viruses: one virus had 11 mutations in the V/P region of the genome; the other, which also contained an extra transcription unit encoding green fluorescent protein (EGFP), had 32 mutations in the N gene. These specific sets of mutations have not been observed in naturally occurring MuV isolates. Unusually, the vast majority of the mutations (48/51) were synonymous. On passage in Vero cells and human B-LCL cells, a B lymphocyte-like cell line, these mutations appear stable as no reversion occurred to the original consensus sequence, although mutations in other parts of the genome occurred and changed in frequency during passage. Defective interfering RNAs accumulate in passage in Vero cells but not in B-LCL cells. Interestingly, in all passaged samples the level of variation in the EGFP gene is the same as in the viral genes, though it is unlikely that this gene is under any functionality constraint. What mechanism gave rise to these viruses with clustered mutations and their stability remains an open question, which is likely of interest to a wider field than mumps reverse genetics.
Item Type: | Articles |
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Additional Information: | The authors have acknowledged funding from a Wellcome Trust grant 101788/Z/13/Z to RER and funding from the respective universities for studentships, Queen’s University Belfast to CGB, and University of St Andrews to EWF. |
Keywords: | Research Article, Biology and life sciences, Medicine and health sciences, Research and analysis methods |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Wignall-fleming, Elizabeth and Bamford, Dr Connor and Randall, Professor Richard and Vattipally, Dr Sreenu |
Creator Roles: | Randall, R.Conceptualization, Supervision, Writing – review and editing Bamford, C.Data curation, Investigation, Methodology, Writing – review and editing Wignall-fleming, E.Data curation, Investigation, Methodology, Software Sreenu, V.Data curation, Software |
Authors: | Bamford, C., Wignall-Fleming, E., Sreenu, V. B., Randall, R., Duprex, P., and Rima, B. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
Journal Name: | PLoS ONE |
Publisher: | Public Library of Science |
ISSN: | 1932-6203 |
ISSN (Online): | 1932-6203 |
Published Online: | 05 July 2019 |
Copyright Holders: | Copyright © 2019 Bamford et al. |
First Published: | First published in PLoS ONE 14(7):e0219168 |
Publisher Policy: | Reproduced under a Creative Commons license |
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