Nenarokova, A., Záhonová, K., Krasilnikova, M., Gahura, O., McCulloch, R. , Zíková, A., Yurchenko, V. and Lukeš, J. (2019) Causes and effects of loss of classical non-homologous end joining pathway in parasitic eukaryotes. mBio, 10(4), e01541-9. (doi: 10.1128/mBio.01541-19) (PMID:31311886) (PMCID:PMC6635534)
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Abstract
We report frequent losses of components of the classical nonhomologous end joining pathway (C-NHEJ), one of the main eukaryotic tools for end joining repair of DNA double-strand breaks, in several lineages of parasitic protists. Moreover, we have identified a single lineage among trypanosomatid flagellates that has lost Ku70 and Ku80, the core C-NHEJ components, and accumulated numerous insertions in many protein-coding genes. We propose a correlation between these two phenomena and discuss the possible impact of the C-NHEJ loss on genome evolution and transition to the parasitic lifestyle.
| Item Type: | Articles |
|---|---|
| Additional Information: | Support from the Czech Grant Agency 18-15962S and the ERD Funds, project OPVVV 16_019/0000759, to J.L. and V.Y., ERC CZ LL1601 to J.L., and the Czech Grant Agency 18-17529S to A.Z. is acknowledged. Work in R.M.’s lab is supported by the BBSRC (BB/K006495/1, BB/M028909/1, and BB/N016165/1) and the Wellcome Trust (206815 and 104111). |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | McCulloch, Professor Richard and Krasilnikova, Marija |
| Authors: | Nenarokova, A., Záhonová, K., Krasilnikova, M., Gahura, O., McCulloch, R., Zíková, A., Yurchenko, V., and Lukeš, J. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | mBio |
| Publisher: | American Society for Microbiology |
| ISSN: | 2150-7511 |
| ISSN (Online): | 2150-7511 |
| Published Online: | 16 July 2019 |
| Copyright Holders: | Copyright © 2019 Nenarokova et al. |
| First Published: | First published in mBio 10(4):e01541-19 |
| Publisher Policy: | Reproduced under a Creative Commons license |
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