Characterisation of the immune-related transcriptome in resected biliary tract cancers

Ghidini, M. et al. (2017) Characterisation of the immune-related transcriptome in resected biliary tract cancers. European Journal of Cancer, 86, pp. 158-165. (doi: 10.1016/j.ejca.2017.09.005) (PMID:28988016) (PMCID:PMC5699791)

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Although biliary tract cancers (BTCs) are known to have an inflammatory component, a detailed characterisation of immune-related transcripts has never been performed. In these studies, nCounter PanCancer Immune Profiling Panel was used to assess the expression of 770 immune-related transcripts in the tumour tissues (TTs) and matched adjacent tissues (ATs) of resected BTCs. Cox regression analysis and Kaplan–Meier methods were used to correlate findings with relapse-free survival (RFS). The first analysis in the TT and AT of an exploratory set (n = 22) showed deregulation of 39 transcripts associated with T-cell activation. Risk of recurrence was associated with a greater number of genes deregulated in AT in comparison to TT. Analysis in the whole set (n = 53) showed a correlation between AT cytotoxic T-lymphocyte antigen-4 (CTLA4) expression and RFS, which maintained statistical significance at multivariate analysis. CTLA4 expression correlated with forkhead box P3 (FOXP3) expression, suggesting enrichment in T regulatory cells. CTLA4 is known to act by binding to the cluster of differentiation 80 (CD80). No association was seen between AT CD80 expression and RFS. However, CD80 expression differentiated prognosis in patients who received adjuvant chemotherapy. We showed that the immunomodulatory transcriptome is deregulated in resected BTCs. Our study includes a small number of patients and does not enable to draw definitive conclusions; however, it provides useful insights into potential transcripts that may deserve further investigation in larger cohorts of patients.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Braconi, Dr Chiara and Valeri, Dr Nicola
Authors: Ghidini, M., Cascione, L., Carotenuto, P., Lampis, A., Trevisani, F., Previdi, M. C., Hahne, J. C., Said-Huntingford, I., Raj, M., Zerbi, A., Mescoli, C., Cillo, U., Rugge, M., Roncalli, M., Torzilli, G., Rimassa, L., Santoro, A., Valeri, N., Fassan, M., and Braconi, C.
College/School:College of Medical Veterinary and Life Sciences > School of Cancer Sciences
Journal Name:European Journal of Cancer
ISSN (Online):1879-0852
Published Online:06 October 2017
Copyright Holders:Copyright © 2017 The Authors
First Published:First published in European Journal of Cancer 86: 158-165
Publisher Policy:Reproduced under a Creative Commons License

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