Kumar, A. et al. (2013) Combination of cytokine responses indicative of latent TB and active TB in Malawian adults. PLoS ONE, 8(11), e79742. (doi: 10.1371/journal.pone.0079742) (PMID:24260295) (PMCID:PMC3832606)
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Abstract
Background: An IFN-γ response to M. tuberculosis-specific antigens is an effective biomarker for M. tuberculosis infection but it cannot discriminate between latent TB infection and active TB disease. Combining a number of cytokine/chemokine responses to M. tuberculosis antigens may enable differentiation of latent TB from active disease. Methods: Asymptomatic recently-exposed individuals (spouses of TB patients) were recruited and tuberculin skin tested, bled and followed-up for two years. Culture supernatants, from a six-day culture of diluted whole blood samples stimulated with M. tuberculosis-derived PPD or ESAT-6, were measured for IFN-γ, IL-10, IL-13, IL-17, TNF-α and CXCL10 using cytokine ELISAs. In addition, 15 patients with sputum smear-positive pulmonary TB were recruited and tested. Results: Spouses with positive IFN-γ responses to M. tuberculosis ESAT-6 (>62.5 pg/mL) and TB patients showed high production of IL-17, CXCL10 and TNF-α. Higher production of IL-10 and IL-17 in response to ESAT-6 was observed in the spouses compared with TB patients while the ratios of IFN-γ/IL-10 and IFN-γ/IL-17 in response to M. tuberculosis-derived PPD were significantly higher in TB patients compared with the spouses. Tuberculin skin test results did not correlate with cytokine responses. Conclusions: CXCL10 and TNF-α may be used as adjunct markers alongside an IFN-γ release assay to diagnose M. tuberculosis infection, and IL-17 and IL-10 production may differentiate individuals with LTBI from active TB.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Crampin, Professor Mia |
Authors: | Kumar, A., Hur, Y.-G., Gorak-Stolinska, P., Ben-Smith, A., Lalor, M. K., Chaguluka, S., Dacombe, R., Doherty, T. M., Ottenhoff, T. H., Dockrell, H. M., and Crampin, A. C. |
College/School: | College of Medical Veterinary and Life Sciences > School of Health & Wellbeing > Public Health |
Journal Name: | PLoS ONE |
Publisher: | Public Library of Science |
ISSN: | 1932-6203 |
ISSN (Online): | 1932-6203 |
Copyright Holders: | Copyright © 2013 Hur et al. |
First Published: | First published in PLoS ONE 8(11):e79742 |
Publisher Policy: | Reproduced under a Creative Commons License |
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