Stewart, M., Krishna, S., Burchmore, R. , Brun, R., de Koning, H. , Boykin, D., Tidwell, R., Hall, J. and Barrett, M. (2005) Detection of arsenical drug resistance in Trypanosoma brucei with a simple fluorescence test. Lancet, 366, pp. 486-487. (doi: 10.1016/S0140-6736(05)66793-1)
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Abstract
The resurgence of human African trypanosomiasis (HAT), coupled with an increased incidence of drug resistance, is of concern. We report a quick, simple, and sensitive test for identification of parasites resistant to melarsoprol, the main drug used to treat late stage HAT. Resistant parasites are defective in a plasma membrane transporter responsible for drug uptake. The same transporter carries the fluorescent diamidine DB99 (2,5-bis-(4-amidinophenyl)-3,4-dimethylfuran) into trypanosomes. The two DNA-containing structures in the trypanosome—the nucleus and the kinetoplast—begin to fluoresce within 1 min of introduction of DB99, unless drug resistant.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Burchmore, Dr Richard and De Koning, Professor Harry and Barrett, Professor Michael |
| Authors: | Stewart, M., Krishna, S., Burchmore, R., Brun, R., de Koning, H., Boykin, D., Tidwell, R., Hall, J., and Barrett, M. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Lancet |
| Publisher: | The Lancet Publishing Group |
| ISSN: | 0140-6736 |
| ISSN (Online): | 1474-547X |
| Related URLs: |
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