Discovery of novel astrovirus and calicivirus identified in ruddy turnstones in Brazil

de Souza, W. M., Fumagalli, M. J., de Araujo, J., Ometto, T., Modha, S. , Thomazelli, L. M., Durigon, E. L., Murcia, P. R. and Figueiredo, L. T. M. (2019) Discovery of novel astrovirus and calicivirus identified in ruddy turnstones in Brazil. Scientific Reports, 9, 5556. (doi: 10.1038/s41598-019-42110-3) (PMID:30944402) (PMCID:PMC6447618)

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Abstract

Birds are the natural reservoir of viruses with zoonotic potential, as well as contributing to the evolution, emergence, and dissemination of novel viruses. In this study, we applied a high-throughput screening approach to identify the diversity of viruses in 118 samples of birds captured between October 2006 to October 2010 in the North and Northeast regions of Brazil. We found nearly complete genomes of novel species of astrovirus and calicivirus in cloacal swabs of ruddy turnstones (Arenaria interpres) collected in Coroa do Avião islet, Pernambuco State. These viruses are positive-sense single-stranded RNA with a genome of ~7 to 8 kb, and were designated as Ruddy turnstone astrovirus (RtAstV) and Ruddy turnstone calicivirus (RTCV), respectively. Phylogenetic analysis showed that RtAstV and RTCV grouped in a monophyletic clade with viruses identified from poultry samples (i.e., chicken, goose, and turkey), including viruses associated with acute nephritis in chickens. Attempts of viral propagation in monkey and chicken cell lines for both viruses were unsuccessful. Also, we found genomes related with viral families that infect invertebrates and plants, suggesting that they might be ingested in the birds' diet. In sum, these findings shed new light on the diversity of viruses in migratory birds with the notable characterization of a novel astrovirus and calicivirus.

Item Type:Articles
Additional Information:This work was supported by the Fundação de Amparo à Pesquisa do Estado de São Paulo, Brazil (Grant Number. 09/05994-9; 11/13821-7; 13/05485-2 and 13/14929-1, and Scholarships No. 12/24150-9; 15/05778-5; 17/13981-0; 16/01414-1 and 18/09383-3). CNPq– process 141981/2006-7. PRM was supported by the Medical Research Council of the United Kingdom (Grant MC_UU_120/14/9).
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Modha, Ms Sejal and Murcia, Dr Pablo
Authors: de Souza, W. M., Fumagalli, M. J., de Araujo, J., Ometto, T., Modha, S., Thomazelli, L. M., Durigon, E. L., Murcia, P. R., and Figueiredo, L. T. M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Scientific Reports
Publisher:Nature Research
ISSN:2045-2322
ISSN (Online):2045-2322
Copyright Holders:Copyright © 2019 The Authors
First Published:First published in Scientific Reports 9:5556
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
709011Quinquennial Core FundsMassimo PalmariniMedical Research Council (MRC)MC_UU_12014/9MVLS III - CENTRE FOR VIRUS RESEARCH