Regulatory T cell-derived extracellular vesicles modify dendritic cell function

Tung, S. L. et al. (2018) Regulatory T cell-derived extracellular vesicles modify dendritic cell function. Scientific Reports, 8, 6065. (doi: 10.1038/s41598-018-24531-8) (PMID:29666503) (PMCID:PMC5904112)

Abstract

Regulatory T cells (Treg) are a subpopulation of T cells that maintain tolerance to self and limit other immune responses. They achieve this through different mechanisms including the release of extracellular vesicles (EVs) such as exosomes as shown by us, and others. One of the ways that Treg derived EVs inhibit target cells such as effector T cells is via the transfer of miRNA. Another key target for the immunoregulatory function of Tregs is the dendritic cells (DCs). In this study we demonstrate directly, and for the first time, that miRNAs are transferred from Tregs to DCs via Treg derived EVs. In particular two miRNAs, namely miR-150-5p and miR-142-3p, were increased in DCs following their interaction with Tregs and Treg derived exosomes. One of the consequences for DCs following the acquisition of miRNAs contained in Treg derived EVs was the induction of a tolerogenic phenotype in these cells, with increased IL-10 and decreased IL-6 production being observed following LPS stimulation. Altogether our findings provide data to support the idea that intercellular transfer of miRNAs via EVs may be a novel mechanism by which Tregs regulate DC function and could represent a mechanism to inhibit immune reactions in tissues.

• Item Type: Articles
• Additional Information: Tis work was supported by a grant from the British Heart Foundation and the Rosetrees Trust. Tis work was also supported by the Department of Health via the National Institute for Health Research Comprehensive Biomedical Research Centre award to Guy’s and St Tomas’ NHS Foundation Trust in partnership with King’s College London and King’s College Hospital NHS Foundation Trust.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Carlin, Dr Leo
• Authors: Tung, S. L., Boardman, D. A., Sen, M., Letizia, M., Peng, Q., Cianci, N., Dioni, L., Carlin, L. M., Lechler, R., Bollati, V.;e., Lombardi, G., and Smyth, L. A.
• College/School: College of Medical Veterinary and Life Sciences > School of Cancer Sciences
• Journal Name: Scientific Reports
• Publisher: Nature Research
• ISSN: 2045-2322
• ISSN (Online): 2045-2322
• Copyright Holders: Copyright © 2018 The Authors
• First Published: First published in Scientific Reports 8: 6065
• Publisher Policy: Reproduced under a Creative Commons License