Damasceno, J. D., Tosi, L. R.O., Santos, R. E.R.S. and Mottram, J. C. (2019) DiCre-based inducible gene expression. In: Clos, J. (ed.) Leishmania: Methods and Protocols. Series: Methods in molecular biology (1971). Humana Press: New York, NY, pp. 225-235. ISBN 9781493992096 (doi: 10.1007/978-1-4939-9210-2_11)
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Abstract
Induction of gene expression is a valuable approach for functional studies since it allows for the assessment of phenotypes without the need for clonal selection. Inducible expression can find a wide range of applications, from the study of essential genes to the characterization of overexpression of genes of interest. Here, we describe a detailed protocol for the use of the DiCre-based inducible gene expression system in Leishmania parasites. This is a tightly regulated induction system that allows for time- and dose-controlled expression of gene products, as rapidly as within 12 h.
| Item Type: | Book Sections |
|---|---|
| Keywords: | Conditional genome engineering, DiCre, dimerizable cre recombinase, inducible gene expression, Leishmania, trypanosomatids. |
| Status: | Published |
| Glasgow Author(s) Enlighten ID: | Damasceno, Dr Jeziel and Mottram, Professor Jeremy |
| Authors: | Damasceno, J. D., Tosi, L. R.O., Santos, R. E.R.S., and Mottram, J. C. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Publisher: | Humana Press |
| ISBN: | 9781493992096 |
| Published Online: | 13 April 2019 |
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