Suárez, N. M. et al. (2019) Human cytomegalovirus genomes sequenced directly from clinical material: variation, multiple-strain infection, recombination and gene loss. Journal of Infectious Diseases, 220(5), pp. 781-791. (doi: 10.1093/infdis/jiz208) (PMID:31050742) (PMCID:PMC6667795)
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Abstract
The genomic characteristics of human cytomegalovirus (HCMV) strains sequenced directly from clinical pathology samples were investigated, focusing on variation, multiple-strain infection, recombination, and gene loss. A total of 207 datasets generated in this and previous studies using target enrichment and high-throughput sequencing were analyzed, in the process enabling the determination of genome sequences for 91 strains. Key findings were that (i) it is important to monitor the quality of sequencing libraries in investigating variation; (ii) many recombinant strains have been transmitted during HCMV evolution, and some have apparently survived for thousands of years without further recombination; (iii) mutants with nonfunctional genes (pseudogenes) have been circulating and recombining for long periods and can cause congenital infection and resulting clinical sequelae; and (iv) intrahost variation in single-strain infections is much less than that in multiple-strain infections. Future population-based studies are likely to continue illuminating the evolution, epidemiology, and pathogenesis of HCMV.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Camiolo, Dr Salvatore and Wilkie, Dr Gavin and Suarez, Dr Nicolas and Hughes, Dr Joseph and Vattipally, Dr Sreenu and Holton, Dr Mary and Davison, Professor Andrew |
| Authors: | Suárez, N. M., Wilkie, G. S., Hage, E., Camiolo, S., Holton, M., Hughes, J., Maabar, M., Sreenu, V. B., Dhingra, A., Gompels, U. A., Wilkinson, G. W.G., Baldanti, F., Furione, M., Lilleri, D., Arossa, A., Ganzenmueller, T., Gerna, G., Hubáček, P., Schulz, T. F., Wolf, D., Zavattoni, M., and Davison, A. J. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
| Journal Name: | Journal of Infectious Diseases |
| Publisher: | Oxford University Press |
| ISSN: | 0022-1899 |
| ISSN (Online): | 1537-6613 |
| Published Online: | 02 May 2019 |
| Copyright Holders: | Copyright © 2019 The Authors |
| First Published: | First published in Journal of Infectious Diseases 220(5):781-791 |
| Publisher Policy: | Reproduced under a Creative Commons license |
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