Hypothermic preservation of red blood cells in different conditions of inert gas xenon: hyperbaria and clathrates

Ponomarev, A., Rodin, V. V., Gurevich, L., Melekhin, V. and Makeev, O. (2018) Hypothermic preservation of red blood cells in different conditions of inert gas xenon: hyperbaria and clathrates. Cryoletters, 39(6), pp. 391-400. (PMID:30963157)

Abstract

Background: Xenon is an inert gas promising for the preservation of biomaterials, which forms clathrate hydrates above 0°C. Objective: This study addresses the effect of hyperbaric xenon (P = 303 kPa) and water-xenon clathrates (P ≥ 608 kPa) on 30 days preservation of red blood cells (RBCs) at +4°C. Materials and Methods: RBCs from healthy human donors were preserved under four different conditions: without preservatives (negative control), in CPDA-1, hyperbaric xenon, and xenon clathrate hydrates. Results: The qualitative (mean RBC volume, anisocytosis degree and mean osmotic fragility) and quantitative characteristics (RBC count and hemolysis degree) of preserved RBCs were measured. Conclusion: The positive role of hyperbaric xenon in the preservation of erythrocytes is attributed to the equilibrium extraction and displacement of O and CO by xenon. The effect is presumably due to a lowering of oxygen concentration and a decrease in the production of reactive oxygen species.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Rodin, Dr Victor
• Authors: Ponomarev, A., Rodin, V. V., Gurevich, L., Melekhin, V., and Makeev, O.
• College/School: College of Medical Veterinary and Life Sciences > School of Psychology & Neuroscience
• Journal Name: Cryoletters
• Publisher: CryoLetters LLP
• ISSN: 0143-2044
• ISSN (Online): 1742-0644