The Role of Toll-Like Receptors (TLR-2 and-4) and Triggering Receptor Expressed on Myeloid Cells 1 (TREM-1) in Human Term and Preterm Labor

Youssef, R., Ledingham, M., Bollapragada, S., O'Gorman, N., Jordan, F., Young, A. and Norman, J. (2009) The Role of Toll-Like Receptors (TLR-2 and-4) and Triggering Receptor Expressed on Myeloid Cells 1 (TREM-1) in Human Term and Preterm Labor. Reproductive Sciences, 16(9), pp. 843-856. (doi: 10.1177/1933719109336621)

Abstract

Objective. To define the role of Toll-like receptors (TLR-2, TLR-4) and triggering receptor expressed on myeloid cells-1 (TREM-1) in pregnant human myometrium. Study Design. Expression of TLR-2 and -4 mRNA and protein and TREM-1 mRNA was quantified in human myometrial samples. Subsequently, we investigated the effect of medroxyprogesterone acetate (MPA) as a functional inhibitor of the TLR agonist lipopolysaccharide (LPS). Results. Messenger RNA expression of TLR-2 and -4 was significantly higher in myometrium at term compared with preterm (P = .009 and .016, respectively). Toll-like receptor-2 protein expression was significantly higher during labor (P = .002) compared with nonlaboring samples. Triggering receptor expressed on myeloid cells-1 mRNA expression was increased in both myometrium and cervix after labor at term (P < .05). Medroxyprogesterone acetate significantly suppressed LPS-induced production of interleukin 1b (IL-1b), IL-6, and IL-8 in vitro (P < .05). Conclusion. Toll-like receptors 2and 4 and TREM-1 are expressed in human myometrium and may play a role in the mechanism of labor at term, and their functional effects are inhibited by MPA.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Youssef, Dr Refaat and Jordan, Mrs Fiona and Ledingham, Dr Marianne and Norman, Professor Jane and Bollapragada, Dr Shrikant
• Authors: Youssef, R., Ledingham, M., Bollapragada, S., O'Gorman, N., Jordan, F., Young, A., and Norman, J.
• College/School: College of Medical Veterinary and Life Sciences
• Journal Name: Reproductive Sciences
• ISSN: 1933-7191