Miller, L. , Greensmith, D.J., Sankaranarayanan, R., O'Neill, S.C. and Eisner, D.A. (2015) The effect of 2,5-di-(tert-butyl)-1,4-benzohydroquinone (TBQ) on intracellular Ca2+ handling in rat ventricular myocytes. Cell Calcium, 58(2), pp. 208-214. (doi: 10.1016/j.ceca.2015.05.002) (PMID:26120055) (PMCID:PMC4509554)
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Abstract
2,5-Di-(tert-butyl)-1,4-benzohydroquinone (TBQ) is a reversible inhibitor of SERCA, potentially making it a useful tool to study the effects of SERCA inhibition in cardiac cells. However, it is unknown if TBQ also has effects on other components of ventricular Ca handling. The aim of these experiments was to characterise the effects of TBQ on Ca handling in rat ventricular myocytes and assess its suitability as a specific inhibitor of SERCA. This was achieved by voltage clamp via perforated patch and [Ca2+]i measurement using Fluo-3 AM. TBQ produced a fully reversible, concentration dependent decrease in the rate of systolic Ca decay. 10 μM TBQ decreased the amplitude of the systolic Ca transient by 48 ± 5% and the rate of decay by 54 ± 6%. SR Ca content was also reduced by 62 ± 4%. However, 10 μM TBQ also decreased the peak L-type Ca current by 23 ± 7%. At higher concentrations (100 μM), TBQ also activated an outward current with a current–voltage relationship consistent with a potassium current. This outward current was abolished by Glibenclamide (100 μM). These data show that TBQ can be used to reversibly inhibit SERCA. However, at concentrations that decrease SERCA activity, TBQ also decreases the L-type Ca current and (at higher concentrations) activates an outward current which appears to be an ATP dependent potassium current. We conclude that TBQ cannot be used as a specific inhibitor of SERCA in rat ventricular myocytes.
| Item Type: | Articles |
|---|---|
| Additional Information: | This work was supported by British Heart Foundation from the following grants BHF RG/06/002/21138, PG/11/16/28777, FS/11/15/28693. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Miller, Dr Louise |
| Authors: | Miller, L., Greensmith, D.J., Sankaranarayanan, R., O'Neill, S.C., and Eisner, D.A. |
| Subjects: | Q Science > QP Physiology |
| College/School: | College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing |
| Journal Name: | Cell Calcium |
| Publisher: | Elsevier |
| ISSN: | 0143-4160 |
| ISSN (Online): | 1532-199 |
| Published Online: | 29 May 2015 |
| Copyright Holders: | Copyright © 2015 The Authors |
| First Published: | First published in Cell Calcium 58(2): 208-214 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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