Kaclíková, E. and Oravcová, K. (2016) Identification of thermotolerant Cronobacter strains using multiplex real-time polymerase chain reaction. Journal of Food and Nutrition Research, 55(3), pp. 278-281.
Full text not currently available from Enlighten.
Abstract
Cronobacter sp. is considered an opportunistic pathogen implicated in food-borne life-threatening infection, in particular in neonates and infants. Contaminated powdered infant formula was identified as the primary source of Cronobacter sp. at very low levels. Multiplication of survived cells of thermotolerant Cronobacter strains was often found a crucial factor for causing outbreaks. The aim of our work was to develop and evaluate a detection method for thermotolerant Cronobacter strains using triplex TaqMan real-time polymerase chain reaction (PCR) targeting a specific sequence for Cronobacter sp., a thermotolerance marker and an internal amplification control. The proposed method was evaluated on 48 strains, including 32 Cronobacter sp. and 16 related species of Enterobacteriaceae family. The results showed 100% inclusivity and exclusivity, and 100% correlation with the time required to kill 90% of cells exposed at 58 °C (D58-value). The developed real-time PCR method facilitated rapid and specific detection of thermotolerant Cronobacter strains isolated from powdered infant formula products or from the production chain.
| Item Type: | Articles |
|---|---|
| Additional Information: | This work is a result of implementation of the project ITMS 26240220089 “Effective control methods for safety foods” of the Agency for the Structural Funds of the European Union, Ministry of Education, Science, Research and Sport of the Slovak Republic. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Oravcova, Dr Katarina |
| Authors: | Kaclíková, E., and Oravcová, K. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
| Journal Name: | Journal of Food and Nutrition Research |
| Publisher: | Vup Food Research Institute |
| ISSN: | 1336-8672 |
| ISSN (Online): | 2333-1119 |
| Published Online: | 26 May 2016 |
| Related URLs: |
University Staff: Request a correction | Enlighten Editors: Update this record
Deposit and Record Details
Deposit and Record Details