Carlin, L. , Eleme, K., McCann, F. E. and Davis, D. M. (2001) Intercellular transfer and supramolecular organization of human leukocyte antigen C at inhibitory natural killer cell immune synapses. Journal of Experimental Medicine, 194(10), pp. 1507-1517. (doi: 10.1084/jem.194.10.1507) (PMID:11714757) (PMCID:PMC2193674)
Full text not currently available from Enlighten.
Abstract
After accumulation of target cell human leukocyte antigen (HLA)-C at inhibitory natural killer (NK) cell immune synapses, some HLA-C transfers from target cells to NK cell plasma membranes and cytoplasm. This unexpected intercellular transfer of HLA-C is dependent on NK receptor recognition, since HLA-Cw6 or -Cw4 but not -Cw3 transfer to an NK transfectant expressing killer Ig-like receptor (KIR)2DL1. Strikingly, live-cell time-lapse laser scanning confocal microscopy shows vesicles containing target cell green fluorescent protein–tagged HLA-C migrating away from immune synapses into NK cells. Unlike clustering of HLA-C at the immune synapse, intercellular transfer of HLA-C is dependent on NK cell ATP, but not target cell ATP. However, the intercellular transfer of HLA-C is not dependent on active polymerization of the actin cytoskeleton. In addition, different arrangements of HLA-C are seen at inhibitory NK immune synapses, and these alter as NK synapses mature, but in a fashion distinct from that seen upon T cell activation.
| Item Type: | Articles |
|---|---|
| Additional Information: | This work is supported by grants from the Medical Research Council, the Biotechnology and Biological Sciences Research Council, and The Royal Society. This article has been corrected. See J. Exp. Med. 2001 Dec 17;194(12):1883. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Carlin, Dr Leo |
| Authors: | Carlin, L., Eleme, K., McCann, F. E., and Davis, D. M. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Cancer Sciences |
| Journal Name: | Journal of Experimental Medicine |
| Publisher: | Rockefeller University Press |
| ISSN: | 0022-1007 |
| ISSN (Online): | 1540-9538 |
University Staff: Request a correction | Enlighten Editors: Update this record
Altmetric
Altmetric