Differential remodeling of a T-cell transcriptome following CD8- versus CD3-induced signaling

Abidi, S. H. I., Dong, T., Vuong, M. T., Sreenu, V. B. , Rowland-Jones, S. L., Evans, E. J. and Davis, S. J. (2008) Differential remodeling of a T-cell transcriptome following CD8- versus CD3-induced signaling. Cell Research, 18(6), pp. 641-648. (doi: 10.1038/cr.2008.56) (PMID:18475290) (PMCID:PMC2731849)

Abstract

CD8 engagement with class I major histocompatibility antigens greatly enhances T-cell activation, but it is not clear how this is achieved. We address the question of whether or not the antibody-mediated ligation of CD8 alone induces transcriptional remodeling in a T-cell clone, using serial analysis of gene expression. Even though it fails to induce overt phenotypic changes, we find that CD8 ligation profoundly alters transcription in the T-cell clone, at a scale comparable to that induced by antibody-mediated ligation of CD3. The character of the resulting changes is distinct, however, with the net effect of CD8 ligation being substantially inhibitory. We speculate that ligating CD8 induces weak, T-cell receptor (TCR)-mediated inhibitory signals reminiscent of the effects of TCR antagonists. Our results imply that CD8 ligation alone is incapable of activating the T-cell clone because it fails to fully induce NFAT-dependent transcription.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Vattipally, Dr Sreenu
• Authors: Abidi, S. H. I., Dong, T., Vuong, M. T., Sreenu, V. B., Rowland-Jones, S. L., Evans, E. J., and Davis, S. J.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity; College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
• Journal Name: Cell Research
• Publisher: Springer Nature
• ISSN: 1001-0602
• ISSN (Online): 1748-7838
• Published Online: 13 May 2008