Molecular cloning and analysis of SSc5D, a new member of the scavenger receptor cysteine-rich superfamily

Gonçalves, C. M. et al. (2009) Molecular cloning and analysis of SSc5D, a new member of the scavenger receptor cysteine-rich superfamily. Molecular Immunology, 46(13), pp. 2585-2596. (doi: 10.1016/j.molimm.2009.05.006) (PMID:19535143)

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Abstract

Glycoproteins of the scavenger receptor cysteine-rich (SRCR) superfamily contain one or more protein modules homologous to the membrane-distal domain of macrophage scavenger receptor I. These domains can be found in the extracellular regions of membrane proteins and in secreted glycoproteins, from the most primitive species to vertebrates. A systematic, bioinformatics-based search for putative human proteins related to the forty-seven known human group B SRCR domains identified a new family member that we have called Soluble Scavenger with 5 Domains (SSc5D). SSc5D is a new soluble protein whose expression is restricted to monocytes/macrophages and T-lymphocytes, and is particularly enriched in the placenta. The gene encoding SSc5D spans 30 kb of genomic DNA, and contains fourteen exons producing a 4.8 kb-long mRNA. The mature polypeptide is predicted to consist of 1573 amino acids comprising, towards the N-terminus, five very similar SRCR domains that are highly conserved among non-marsupial mammals, and a large (>250 nm), very heavily glycosylated, mucin-like sequence towards the C-terminus. Each of the SRCR domains is encoded by a single exon, and contains eight cysteine residues, as observed for all other group B SRCR domains. A shorter isoform encoded by a weakly expressed, alternatively spliced transcript, which lacks the mucin-like C-terminal region, was also identified. It seems likely that SSc5D has a role at the interface between adaptive and innate immunity, or in placental function.

Item Type:Articles
Additional Information:This work was supported by Fundac¸ ão para a Ciência e a Tecnologia, grants PTDC/SAU-MII/64247/2006 and PTDC/SAUGMG/72168/2006, co-funded by the European Regional Development Fund (FEDER). M.A.A.C. was supported by a post-doctoral fellowship from Fundac¸ ão para a CiênciaeaTecnologia (FCT) - Programa Operacional Sociedade da Informac¸ ão (POSI), C.O. is funded by Programa Ciência 2007, C.M.G., T.H., M.I.O. and H.C.P. were recipients of studentships from FCT. V.B.S., E.J.E and S.J.D. are funded by the Wellcome Trust.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Vattipally, Dr Sreenu
Authors: Gonçalves, C. M., Castro, M. A.A., Henriques, T., Oliveira, M. I., Pinheiro, H. C., Oliveira, C., Sreenu, V. B., Evans, E. J., Davis, S. J., Moreira, A., and Carmo, A. M.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
Journal Name:Molecular Immunology
Publisher:Elsevier
ISSN:0161-5890
ISSN (Online):1872-9142
Published Online:16 June 2009

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