TOPs and their regulation

Hamilton, T.L., Stoneley, M., Spriggs, K.A. and Bushell, M. (2006) TOPs and their regulation. Biochemical Society Transactions, 34(1), pp. 12-16. (doi: 10.1042/BST0340012) (PMID:16246169)

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Upon cell-cycle arrest or nutrient deprivation, the cellular rate of ribosome production is reduced significantly. In mammalian cells, this effect is achieved in part through a co-ordinated inhibition of RP (ribosomal protein) synthesis. More specifically, translation initiation on RP mRNAs is inhibited. Translational regulation of RP synthesis is dependent on cis-elements within the 5′-UTRs (5′-untranslated regions) of the RP mRNAs. In particular, a highly conserved 5′-TOP (5′-terminal oligopyrimidine tract) appears to play a key role in the regulation of RP mRNA translation. This article explores recent developments in our understanding of the mechanism of TOP mRNA regulation, focusing on upstream signalling pathways and trans-acting factors, and highlighting some interesting observations which have come to light following the recent development of cDNA microarray technology coupled with polysome analysis.

Item Type:Articles
Additional Information:This work was supported by the BBSRC (Biotechnology and Biological Sciences Research Council) (to M.B., a David Phillips Fellowship, and T.H.) and the Wellcome Trust (M.S. and K.S).
Glasgow Author(s) Enlighten ID:Bushell, Professor Martin
Authors: Hamilton, T.L., Stoneley, M., Spriggs, K.A., and Bushell, M.
College/School:College of Medical Veterinary and Life Sciences > School of Cancer Sciences
Journal Name:Biochemical Society Transactions
Publisher:Portland Press for The Biochemical Society
ISSN (Online):1470-8752

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