Dahal, L. N., Basu, N. , Youssef, H., Khanolkar, R. C., Barker, R. N., Erwig, L. P. and Ward, F. J. (2016) Immunoregulatory soluble CTLA-4 modifies effector T-cell responses in systemic lupus erythematosus. Arthritis Research and Therapy, 18(1), 180. (doi: 10.1186/s13075-016-1075-1)
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Abstract
Background: The inhibitory CTLA-4 molecule is a crucial regulator of immune responses and a target for therapeutic intervention in both autoimmunity and cancer. In particular, CTLA-4 is important in controlling antigen-specific immunity, including responses to autoantigens associated with autoimmune disease. Here, we investigate cytokine responses to a range of lupus-associated autoantigens and assess whether the alternatively spliced isoform of CTLA-4, soluble CTLA-4 (sCTLA-4), contributes to immune regulation of autoantigen-specific immunity in systemic lupus erythematosus (SLE). Methods: The cell culture supernatant production of sCTLA-4 as well as the cytokines IL-10, IFN-γ, and IL-17 from peripheral blood mononuclear cells (PBMC) from lupus patients and age- and sex-matched healthy volunteer donors were measured in response to previously identified histone and small nuclear ribonucleoprotein (snRNP) autoantigen-derived peptides (H391-105, H471-93, and U170K131-151) by ELISA. We also examined the functional contribution of sCTLA-4 to immune regulation in the context of these autoantigenic peptides following blockade of sCTLA-4 with a selective anti-sCTLA-4 monoclonal antibody, JMW-3B3. Results: We identified responses to autoantigenic peptides, which revealed qualitative differences in cytokine (IL-10, IL-17, and IFN-γ) profiles between SLE patients and healthy donors. PBMC from healthy donors responded to each of the lupus peptides by secreting IFN-γ and IL-17, but PBMC from SLE patients produced IL-10. Although we did not observe differences in the levels of serum or PBMC culture supernatant sCTLA-4 in either cohort, blockade of sCTLA-4 in PBMC cultures responding to antigen enhanced the cytokine profiles associated with each group. Conclusion: The results show that lupus autoantigen-derived peptides display varied immunogenicity in lupus versus healthy volunteer donors, while sCTLA-4 acts to regulate the T-cell activity independently of response profile.
Item Type: | Articles |
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Additional Information: | This work was supported by Arthritis Research UK (Grant no. 19282). |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Basu, Professor Neil |
Authors: | Dahal, L. N., Basu, N., Youssef, H., Khanolkar, R. C., Barker, R. N., Erwig, L. P., and Ward, F. J. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
Journal Name: | Arthritis Research and Therapy |
Publisher: | BioMed Central |
ISSN: | 1478-6354 |
ISSN (Online): | 1478-6362 |
Copyright Holders: | Copyright © 2016 The Authors |
First Published: | First published in Arthritis Research and Therapy 18(1):180 |
Publisher Policy: | Reproduced under a Creative Commons License |
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