Novel anchorage of GluR2/3 to the Postsynaptic Density by the AMPA Receptor–Binding Protein ABP

Srivastava, S. et al. (1998) Novel anchorage of GluR2/3 to the Postsynaptic Density by the AMPA Receptor–Binding Protein ABP. Neuron, 21(3), pp. 581-591. (doi: 10.1016/S0896-6273(00)80568-1) (PMID:9768844)

Full text not currently available from Enlighten.


We report the cloning of α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptor–binding protein (ABP), a postsynaptic density (PSD) protein related to glutamate receptor–interacting protein (GRIP) with two sets of three PDZ domains, which binds the GluR2/3 AMPA receptor subunits. ABP exhibits widespread CNS expression and is found at the postsynaptic membrane. We show that the protein interactions of the ABP/GRIP family differ from the PSD-95 family, which binds N-methyl-D-aspartate (NMDA) receptors. ABP binds to the GluR2/3 C–terminal VKI-COOH motif via class II hydrophobic PDZ interactions, distinct from the class I PSD-95–NMDA receptor interaction. ABP and GRIP also form homo- and heteromultimers through PDZ–PDZ interactions but do not bind PSD-95. We suggest that the ABP/GRIP and PSD-95 families form distinct scaffolds that anchor, respectively, AMPA and NMDA receptors.

Item Type:Articles
Additional Information:We thank S. Heineman and J. Boulter for AMPA receptor cDNA plasmids, M. Sheng for the PSD-95 cDNA clone, R. MacKinnon for the PSD-95 PDZ3 crystal structure file, and T. Serra for preparation of the manuscript. Computing was supported by the New York University Research Computing Resource. This work was supported by Department of Energy grants DE-GF02–96ER6226 (to R. A.) and NS29879 (to R. J. W.) and by the Howard Hughes Medical Institute. S. S. and B. S. were supported by National Institutes of Health grant AG13620 (to E. B. Z). R. A. is a member of the Skirball Institute of New York University Medical Center. P. O., F. S. V., G. I., C. D., and S. DeS. are Associates and E. B. Z. is an Investigator of the Howard Hughes Medical Institute.
Glasgow Author(s) Enlighten ID:Inman, Professor Gareth
Authors: Srivastava, S., Osten, P., Vilim, F.S., Khatri, L., Inman, G., States, B., Daly, C., DeSouza, S., Abagyan, R., Valtschanoff, J.G., Weinberg, R..J., and Ziff, E.B.
College/School:College of Medical Veterinary and Life Sciences > School of Cancer Sciences
Journal Name:Neuron
ISSN (Online):1097-4199

University Staff: Request a correction | Enlighten Editors: Update this record