Analysis of foetal expression sites of human type II DNA topoisomerase α and β mRNAs by in situ hybridisation

Zandvliet, D. W.J., Hanby, A. M., Austin, C. A., Marsh, K. L. , Clark, I. B.N., Wright, N. A. and Poulsom, R. (1996) Analysis of foetal expression sites of human type II DNA topoisomerase α and β mRNAs by in situ hybridisation. Biochimica et Biophysica Acta: Gene Structure and Expression, 1307(2), pp. 239-247. (doi:10.1016/0167-4781(96)00063-2) (PMID:8679710)

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Abstract

We present the first analysis of the sites of expression of DNA topoisomerase IIα and IIβ mRNAs in human foetal tissues by in situ hybridisation, using 35S-radiolabelled probes. This revealed differential localisation of topoisomerase IIα and IIβ mRNAs in a range of foetal organs, including foetal kidney (developing structures within the neogenic zone), brain (cortical layers), small intestine (crypt epithelium and muscle), liver (hepatocytes), lung (smooth muscle, and epithelium in the lining of primitive lung buds) and placenta (trophoblastic epithelium). The intensity of expression of topoisomerase IIα mRNA appeared higher than that of topoisomerase IIβ, although topoisomerase IIβ was expressed in a broader range of cell types. The distinct patterns of expression of topoisomerase IIα and β mRNAs indicate differential regulation of these genes, suggesting that the two isoforms may play important but different roles in foetal development, with topoisomerase IIα being expressed most strongly in zones of proliferation.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:West, Dr Katherine
Authors: Zandvliet, D. W.J., Hanby, A. M., Austin, C. A., Marsh, K. L., Clark, I. B.N., Wright, N. A., and Poulsom, R.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
College of Medical Veterinary and Life Sciences > School of Life Sciences
Journal Name:Biochimica et Biophysica Acta: Gene Structure and Expression
Publisher:Elsevier
ISSN:0167-4781
ISSN (Online):1879-2634

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