Genome hypermobility by lateral transduction

Chen, J., Quiles-Puchalt, N., Chiang, Y. N., Bacigalupe, R., Fillol-Salom, A., Chee, M. S. J., Fitzgerald, J. R. and Penadés, J. R. (2018) Genome hypermobility by lateral transduction. Science, 362(6411), pp. 207-212. (doi: 10.1126/science.aat5867) (PMID:30309949)

Abstract

Genetic transduction is a major evolutionary force that underlies bacterial adaptation. Here we report that the temperate bacteriophages of Staphylococcus aureus engage in a distinct form of transduction we term lateral transduction. Staphylococcal prophages do not follow the previously described excision-replication-packaging pathway but instead excise late in their lytic program. Here, DNA packaging initiates in situ from integrated prophages, and large metameric spans including several hundred kilobases of the S. aureus genome are packaged in phage heads at very high frequency. In situ replication before DNA packaging creates multiple prophage genomes so that lateral-transducing particles form during normal phage maturation, transforming parts of the S. aureus chromosome into hypermobile regions of gene transfer.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Penades, Prof Jose R
• Authors: Chen, J., Quiles-Puchalt, N., Chiang, Y. N., Bacigalupe, R., Fillol-Salom, A., Chee, M. S. J., Fitzgerald, J. R., and Penadés, J. R.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity
• Journal Name: Science
• Publisher: American Association for the Advancement of Science
• ISSN: 0036-8075
• ISSN (Online): 1095-9203
• Copyright Holders: Copyright © 2018 The Authors
• First Published: First published in Science 362(6411):207-212
• Publisher Policy: Reproduced in accordance with the copyright policy of the publisher

Project Code	Award No	Project Name	Principal Investigator	Funder's Name	Funder Ref	Lead Dept
66077	2	Molecular biology of the PICIs, a novel and widespread family of mobile genetic elements involved in bacterial virulence	Jose R Penades	Medical Research Council (MRC)	MR/M003876/1	RI INFECTION IMMUNITY & INFLAMMATION
69378	1	Understanding a novel mechanim involving pathogenity islands in the transfer of unlinked chromosomal virulence genes	Jose R Penades	Biotechnology and Biological Sciences Research Council (BBSRC)	BB/N002873/1	RI INFECTION IMMUNITY & INFLAMMATION
68770	1	DUT-Signal	Jose R Penades	European Research Council (ERC)	670932	RI INFECTION IMMUNITY & INFLAMMATION
72724	1	Understanding bacterial host adaptation to combat infectious diseases	Jose R Penades	Wellcome Trust (WELLCOTR)	201531/Z/16/Z	RI INFECTION IMMUNITY & INFLAMMATION