Surface-modified complex SU-8 microstructures for indirect optical manipulation of single cells

Aekbote, B. L. , Fekete, T., Jacak, J., Vizsnyiczai, G., Ormos, P. and Kelemen, L. (2016) Surface-modified complex SU-8 microstructures for indirect optical manipulation of single cells. Biomedical Optics Express, 7(1), pp. 45-56. (doi: 10.1364/BOE.7.000045) (PMID:26819816) (PMCID:PMC4722909)

Abstract

We introduce a method that combines two-photon polymerization (TPP) and surface functionalization to enable the indirect optical manipulation of live cells. TPP-made 3D microstructures were coated specifically with a multilayer of the protein streptavidin and non-specifically with IgG antibody using polyethylene glycol diamine as a linker molecule. Protein density on their surfaces was quantified for various coating methods. The streptavidin-coated structures were shown to attach to biotinated cells reproducibly. We performed basic indirect optical micromanipulation tasks with attached structure-cell couples using complex structures and a multi-focus optical trap. The use of such extended manipulators for indirect optical trapping ensures to keep a safe distance between the trapping beams and the sensitive cell and enables their 6 degrees of freedom actuation.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Aekbote, Dr Badri
• Authors: Aekbote, B. L., Fekete, T., Jacak, J., Vizsnyiczai, G., Ormos, P., and Kelemen, L.
• College/School: College of Science and Engineering > School of Engineering > Biomedical Engineering
• Journal Name: Biomedical Optics Express
• Publisher: Optical Society of America
• ISSN: 2156-7085
• ISSN (Online): 2156-7085
• Published Online: 07 December 2015