Heterogeneity in pure microbial systems: experimental measurements and modeling

González-Cabaleiro, R. , Mitchell, A. M., Smith, W., Wipat, A. and Ofiţeru, I. D. (2017) Heterogeneity in pure microbial systems: experimental measurements and modeling. Frontiers in Microbiology, 8, 1813. (doi: 10.3389/fmicb.2017.01813) (PMID:28970826) (PMCID:PMC5609101)

Abstract

Cellular heterogeneity influences bioprocess performance in ways that until date are not completely elucidated. In order to account for this phenomenon in the design and operation of bioprocesses, reliable analytical and mathematical descriptions are required. We present an overview of the single cell analysis, and the mathematical modeling frameworks that have potential to be used in bioprocess control and optimization, in particular for microbial processes. In order to be suitable for bioprocess monitoring, experimental methods need to be high throughput and to require relatively short processing time. One such method used successfully under dynamic conditions is flow cytometry. Population balance and individual based models are suitable modeling options, the latter one having in particular a good potential to integrate the various data collected through experimentation. This will be highly beneficial for appropriate process design and scale up as a more rigorous approach may prevent a priori unwanted performance losses. It will also help progressing synthetic biology applications to industrial scale.

• Item Type: Articles
• Additional Information: AM acknowledges the support of the EPSRC DTA scholarship. RG-C, AW, and IO acknowledge the support of the EPSRC Frontier Grant “Simulation of open engineered biological systems,” led by Newcastle University, ref EP/K038648. WS and AW acknowledge the support of the EPSRC Grant “Synthetic Portabolomics: Leading the way at the crossroads of the Digital and the Bio Economies,” ref EP/R003629/1.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Gonzalez-Cabaleiro, Dr Rebeca
• Authors: González-Cabaleiro, R., Mitchell, A. M., Smith, W., Wipat, A., and Ofiţeru, I. D.
• College/School: College of Science and Engineering > School of Engineering > Infrastructure and Environment
• Journal Name: Frontiers in Microbiology
• Publisher: Frontiers
• ISSN: 1664-302X
• ISSN (Online): 1664-302X
• Copyright Holders: Copyright © 2017 González-Cabaleiro, Mitchell, Smith, Wipat and Ofiţeru
• First Published: First published in Frontiers in Microbiology 8: 1813
• Publisher Policy: Reproduced under a Creative Commons License