High-speed extended-volume blood flow measurement using engineered point-spread function

Zhou, Y. , Zickus, V., Zammit, P., Taylor, J. M. and Harvey, A. R. (2018) High-speed extended-volume blood flow measurement using engineered point-spread function. Biomedical Optics Express, 9(12), pp. 6444-6454. (doi: 10.1364/BOE.9.006444)

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Experimental characterization of blood flow in living organisms is crucial for understanding the development and function of cardiovascular systems, but there has been no technique reported for snapshot imaging of thick samples in large volumes with high precision. We have combined computational microscopy and the diffraction-free, self-bending property of Airy-beams to track fluorescent beads with sub-micron precision through an extended axial range (up to 600 \textmu m) within the flowing blood of 3 days post-fertilization (dpf) zebrafish embryos. The spatial trajectories of the tracer beads within flowing blood were recorded during transit through both cardinal and intersegmental vessels, and the trajectories were found to be consistent with the segmentation of the vasculature recorded using selective-plane illumination microscopy (SPIM). This method provides sufficiently precise spatial and temporal measurement of 3D blood flow that has the potential for directly probing key biomechanical quantities such as wall shear stress, as well as exploring the fluidic repercussions of cardiovascular diseases. Although we demonstrate the technique for blood flow, the ten-fold better enhancement in the depth range offers improvements in a wide range of applications of high-speed precision measurement of fluid flow, from microfluidics through measurement of cell dynamics to macroscopic aerosol characterizations.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Taylor, Dr Jonathan and Zhou, Mr Yongzhuang and Zammit, Mr Paul and Harvey, Professor Andy
Authors: Zhou, Y., Zickus, V., Zammit, P., Taylor, J. M., and Harvey, A. R.
College/School:College of Science and Engineering > School of Physics and Astronomy
Journal Name:Biomedical Optics Express
Publisher:Optical Society of America
ISSN (Online):2156-7085
Published Online:26 November 2018
Copyright Holders:Copyright © 2018 The Optical Society
First Published:First published in Biomedical Optics Express 9(12): 6444-6454
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
642161Development and optimisation of synchronised 3D in-vivo imaging of the embryonic and juvenile zebrafish heartJonathan TaylorBritish Heart Foundation (BHF)NH/14/2/31074S&E P&A - PHYSICS & ASTRONOMY
678901EPSRC DTG 2014Mary Beth KneafseyEngineering and Physical Sciences Research Council (EPSRC)EP/M506539/1R&I - RESEARCH STRATEGY & INNOVATION
608951Engineering and Physical Sciences Doctoral Training Grant 2012-16Mary Beth KneafseyEngineering and Physical Sciences Research Council (EPSRC)EP/K503058/1VPO VICE PRINCIPAL RESEARCH & ENTERPRISE