Interactome comparison of human embryonic stem cell lines with the inner cell mass and trophectoderm

Stevens, A., Smith, H., Garner, T., Minogue, B., Sneddon, S. , Shaw, L., Oldershaw, R., Bates, N., Brison, D. R. and Kimber, S. J. (2018) Interactome comparison of human embryonic stem cell lines with the inner cell mass and trophectoderm. bioRxiv, (doi: 10.1101/411439)

Abstract

Networks of interacting co-regulated genes distinguish the inner cell mass (ICM) from the differentiated trophectoderm (TE) in the preimplantation blastocyst, in a species specific manner. In mouse the ground state pluripotency of the ICM appears to be maintained in murine embryonic stem cells (ESCs) derived from the ICM. This is not the case for human ESCs. In order to gain insight into this phenomenon, we have used quantitative network analysis to identify how similar human (h)ESCs are to the human ICM. Using the hESC lines MAN1, HUES3 and HUES7 we have shown that all have only a limited overlap with ICM specific gene expression, but that this overlap is enriched for network properties that correspond to key aspects of function including transcription factor activity and the hierarchy of network modules. These analyses provide an important framework which highlights the developmental origins of hESCs.

• Item Type: Articles
• Additional Information: This article is a preprint and has not been peer-reviewed.
• Status: Published
• Refereed: No
• Glasgow Author(s) Enlighten ID: Sneddon, Dr Sharon
• Authors: Stevens, A., Smith, H., Garner, T., Minogue, B., Sneddon, S., Shaw, L., Oldershaw, R., Bates, N., Brison, D. R., and Kimber, S. J.
• Subjects: R Medicine > R Medicine (General)
• College/School: College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing
• Journal Name: bioRxiv
• Publisher: Cold Spring Harbor Laboratory
• Copyright Holders: Copyright © 2018 The Authors
• Publisher Policy: Reproduced under a Creative Commons License

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