Liu, C., Emami, S. N., Pettersson, J., Ranford-Cartwright, L. , Faye, I. and Parmryd, I. (2018) Vγ9Vδ2 T cells proliferate in response to phosphoantigens released from erythrocytes infected with asexual and gametocyte stage Plasmodium falciparum. Cellular Immunology, 334, pp. 11-19. (doi: 10.1016/j.cellimm.2018.08.012) (PMID:30177348)
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Abstract
Vγ9Vδ2 T cells, the dominant γδ T cell subset in human peripheral blood, are stimulated by phosphoantigens, of which (E)-4-Hydroxy-3-methyl-but-2-enyl pyrophosphate, is produced in the apicoplast of malaria parasites. Cell-free media from synchronised Plasmodium falciparum asexual ring, trophozoite, and schizont stage-cultures of high purity as well as media from ruptured schizont cultures, all stimulated Vγ9Vδ2 T cell proliferation, as did media from pure gametocyte cultures, whereas media from uninfected erythrocytes cultures did not. The media from ruptured schizont cultures and all the asexual and gametocyte stage cultures contained only background iron levels, suggesting that all erythrocyte haemoglobin is consumed as the parasites develop and supporting that the phosphoantigens were released from intact parasitized erythrocytes. The Vγ9Vδ2 T cell-stimulating agent was not affected by freezing, thawing or heating but was sensitive to phosphatase treatment, confirming its phosphoantigen identity. In summary, phosphoantigens are released from parasitised erythrocytes at all developmental blood stages.
| Item Type: | Articles |
|---|---|
| Additional Information: | This work was supported by grants to IP from the Swedish Research Council (Dnr 2010-3258), AFA Insurance and Clas Groschinsky’s Foundation and a grant to IF from INFRAVEC (EU/FP7). |
| Keywords: | Asexual blood stages, gametocytes, iron, malaria, phospoantigens, Plasmodium falciparum, Vγ9Vδ2 T cells. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Ranford-Cartwright, Dr Lisa |
| Authors: | Liu, C., Emami, S. N., Pettersson, J., Ranford-Cartwright, L., Faye, I., and Parmryd, I. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
| Journal Name: | Cellular Immunology |
| Publisher: | Elsevier |
| ISSN: | 0008-8749 |
| ISSN (Online): | 1090-2163 |
| Published Online: | 25 August 2018 |
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