Multilayered nanoplasmonic arrays for self-referenced biosensing

Sperling, J. R. , Macias, G., Neale, S. L. and Clark, A. W. (2018) Multilayered nanoplasmonic arrays for self-referenced biosensing. ACS Applied Materials and Interfaces, 10(40), pp. 34774-34780. (doi: 10.1021/acsami.8b12604) (PMID:30207457)

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Nanostructured sensors based on localized surface plasmon resonance (LSPR) offer a number of advantages over other optical sensing technologies, making them excellent candidates for miniaturized, label-free chemical and biological detection. Highly sensitive to local refractive index changes, the resonance peaks of the nanosensors shift by different amounts when subject to different biological and chemical environments. Modifications to the nanostructure surface allow for the detection of specific molecules and chemicals with shifts so sensitive that the presence of single molecules can be detected. However, this extreme sensitivity has its drawbacks. Resonance shifts also occur because of temperature shifts, light-intensity fluctuations, and other environmental factors. To distinguish detection from drift, a secondary sensor region is often required. This often doubles the size of the device, requires two light sources and detectors (or complex optics), doubles the sample volume required (which may be expensive, or may not be possible if the sample quantity is limited), and subjects the reference to potential biofouling. Here, we present a new proof-of-concept multilayered LSPR sensor design that incorporates both a sensing layer and an encapsulated reference layer within the same region. By doing so, we are able to monitor and correct for sensor drift without the need for a secondary reference channel. We demonstrate the suitability of this sensor for sucrose concentration measurements and for the detection of biotin–avidin interactions, while also showing that the sensor can self-correct for drift. We believe that this multilayer sensor design holds promise for point-of-care diagnostics.

Item Type:Articles
Additional Information:Also supported by The Leverhulme Trust (grant RPG-2014-343).
Glasgow Author(s) Enlighten ID:Neale, Professor Steven and Macias Sotuela, Dr Gerard and Clark, Professor Alasdair and Sperling, Dr Justin
Authors: Sperling, J. R., Macias, G., Neale, S. L., and Clark, A. W.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
College of Science and Engineering > School of Engineering > Systems Power and Energy
Journal Name:ACS Applied Materials and Interfaces
Publisher:American Chemical Society
ISSN (Online):1944-8252
Published Online:12 September 2018
Copyright Holders:Copyright © 2018 The Authors
First Published:First published in ACS Applied Materials and Interfaces 10(40): 34774-34780
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
749331EPSRC-ISF 2016: UV illuminator for DNA-origami & Micro-spectrometer for monitoring the biological assembly of plasmonic metasurfaces in real-timeAlasdair ClarkEngineering and Physical Sciences Research Council (EPSRC)EP/P51133X/1ENG - BIOMEDICAL ENGINEERING
704621DNA-directed construction of three-dimensional photosynthetic assembliesAlasdair ClarkBiotechnology and Biological Sciences Research Council (BBSRC)BB/N016734/1ENG - BIOMEDICAL ENGINEERING