Patil, S. B., Dheeman, D. S., Al-Rawhani, M. A., Velugotla, S. , Nagy, B., Cheah, B. C. , Grant, J. P. , Accarino, C., Barrett, M. P. and Cumming, D. R.S. (2018) An integrated portable system for single chip simultaneous measurement of multiple disease associated metabolites. Biosensors and Bioelectronics, 122, pp. 88-94. (doi: 10.1016/j.bios.2018.09.013) (PMID:30245326)
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Abstract
Metabolites, the small molecules that underpin life, can act as indicators of the physiological state of the body when their abundance varies, offering routes to diagnosis of many diseases. The ability to assay for multiple metabolites simultaneously will underpin a new generation of precision diagnostic tools. Here, we report the development of a handheld device based on complementary metal oxide semiconductor (CMOS) technology with multiple isolated micro-well reaction zones and integrated optical sensing allowing simultaneous enzyme-based assays of multiple metabolites (choline, xanthine, sarcosine and cholesterol) associated with multiple diseases. These metabolites were measured in clinically relevant concentration range with minimum concentrations measured: 25 μM for choline, 100 μM for xanthine, 1.25 μM for sarcosine and 50 μM for cholesterol. Linking the device to an Android-based user interface allows for quantification of metabolites in serum and urine within 2 min of applying samples to the device. The quantitative performance of the device was validated by comparison to accredited tests for cholesterol and glucose.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Al-Rawhani, Dr Mohammed and Accarino, Claudio and Velugotla, Dr Srinivas and Cumming, Professor David and Patil, Dr Samadhan and Cheah, Dr Boon Chong and Grant, Dr James and Nagy, Mr Bence and Barrett, Professor Michael and Dheeman, Dr Dharmendra |
Authors: | Patil, S. B., Dheeman, D. S., Al-Rawhani, M. A., Velugotla, S., Nagy, B., Cheah, B. C., Grant, J. P., Accarino, C., Barrett, M. P., and Cumming, D. R.S. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Science and Engineering > School of Engineering > Electronics and Nanoscale Engineering |
Journal Name: | Biosensors and Bioelectronics |
Publisher: | Elsevier |
ISSN: | 0956-5663 |
ISSN (Online): | 1873-4235 |
Published Online: | 05 September 2018 |
Copyright Holders: | Copyright © 2018 The Authors |
First Published: | First published in Biosensors and Bioelectronics 122:88-94 |
Publisher Policy: | Reproduced under a Creative Commons License |
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