Hydra mesoglea proteome identifies thrombospondin as a conserved component active in head organizer restriction

Lommel, M. et al. (2018) Hydra mesoglea proteome identifies thrombospondin as a conserved component active in head organizer restriction. Scientific Reports, 8, 11753. (doi: 10.1038/s41598-018-30035-2) (PMID:30082916) (PMCID:PMC6079037)

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Abstract

Thrombospondins (TSPs) are multidomain glycoproteins with complex matricellular functions in tissue homeostasis and remodeling. We describe a novel role of TSP as a Wnt signaling target in the basal eumetazoan Hydra. Proteome analysis identified Hydra magnipapillata TSP (HmTSP) as a major component of the cnidarian mesoglea. In general, the domain organization of cnidarian TSPs is related to the pentameric TSPs of bilaterians, and in phylogenetic analyses cnidarian TSPs formed a separate clade of high sequence diversity. HmTSP expression in polyps was restricted to the hypostomal tip and tentacle bases that harbor Wnt-regulated organizer tissues. In the hypostome, HmTSP- and Wnt3-expressing cells were identical or in close vicinity to each other, and regions of ectopic tentacle formation induced by pharmacological β-Catenin activation (Alsterpaullone) corresponded to foci of HmTSP expression. Chromatin immunoprecipitation (ChIP) confirmed binding of Hydra TCF to conserved elements in the HmTSP promotor region. Accordingly, β-Catenin knockdown by siRNAs reduced normal HmTSP expression at the head organizer. In contrast, knockdown of HmTSP expression led to increased numbers of ectopic organizers in Alsterpaullone-treated animals, indicating a negative regulatory function. Our data suggest an unexpected role for HmTSP as a feedback inhibitor of Wnt signaling during Hydra body axis patterning and maintenance.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Thomson, Dr Drew
Authors: Lommel, M., Strompen, J., Hellewell, A. L., Balasubramanian, G. P., Christofidou, E. D., Thomson, A. R., Boyle, A. L., Woolfson, D. N., Puglisi, K., Hartl, M., Holstein, T. W., Adams, J. C., and Özbek, S.
College/School:College of Science and Engineering > School of Chemistry
Journal Name:Scientific Reports
Publisher:Nature Research
ISSN:2045-2322
ISSN (Online):2045-2322
Copyright Holders:Copyright © 2018 The Authors
First Published:First published in Scientific Reports 8: 11753
Publisher Policy:Reproduced under a Creative Commons License

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