Bacteria-based materials for stem cell engineering

Hay, J. J., Rodrigo-Navarro, A. , Petaroudi, M., Bryksin, A. V., Garcia, A. J., Barker, T. H., Dalby, M. J. and Salmeron-Sanchez, M. (2018) Bacteria-based materials for stem cell engineering. Advanced Materials, 30(43), 1804310. (doi: 10.1002/adma.201804310) (PMID:30209838)

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Materials can be engineered to deliver specific biological cues that control stem cell growth and differentiation. However, current materials are still limited for stem cell engineering as stem cells are regulated by a complex biological milieu that requires spatiotemporal control. Here a new approach of using materials that incorporate designed bacteria as units that can be engineered to control human mesenchymal stem cells (hMSCs), in a highly dynamic‐temporal manner, is presented. Engineered Lactococcus lactis spontaneously colonizes a variety of material surfaces (e.g., polymers, metals, and ceramics) and is able to maintain growth and induce differentiation of hMSCs in 2D/3D surfaces and hydrogels. Controlled, dynamic, expression of fibronectin fragments supports stem cell growth, whereas inducible‐temporal regulation of secreted bone morphogenetic protein‐2 drives osteogenesis in an on‐demand manner. This approach enables stem cell technologies using material systems that host symbiotic interactions between eukaryotic and prokaryotic cells.

Item Type:Articles
Additional Information:Also supported by the European Research Council (ERC306990).
Glasgow Author(s) Enlighten ID:Hay, Mr Jake and Salmeron-Sanchez, Professor Manuel and Dalby, Professor Matthew and Rodrigo-Navarro, Mr Aleixandre and Petaroudi, Dr Michaela
Authors: Hay, J. J., Rodrigo-Navarro, A., Petaroudi, M., Bryksin, A. V., Garcia, A. J., Barker, T. H., Dalby, M. J., and Salmeron-Sanchez, M.
College/School:College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Advanced Materials
ISSN (Online):1521-4095
Published Online:12 September 2018
Copyright Holders:Copyright © 2018 The Authors
First Published:First published in Advanced Materials 30(43): 1804310
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
722061Engineering growth factor microenvironments- a new therapeutic paradigm for regenerative medicineManuel Salmeron-SanchezEngineering and Physical Sciences Research Council (EPSRC)EP/P001114/1ENG - BIOMEDICAL ENGINEERING
691041Living interfaces based on non-pathogenic bacterial to control stem cell differentiationManuel Salmeron-SanchezLeverhulme Trust (LEVERHUL)RPG-2015-191ENG - BIOMEDICAL ENGINEERING