Merging in-solution x-ray and neutron scattering data allows fine structural analysis of membrane–protein detergent complexes

Dias Mirandela, G. et al. (2018) Merging in-solution x-ray and neutron scattering data allows fine structural analysis of membrane–protein detergent complexes. Journal of Physical Chemistry Letters, 9(14), pp. 3910-3914. (doi: 10.1021/acs.jpclett.8b01598) (PMID:29939747)

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Abstract

In-solution small-angle X-ray and neutron scattering (SAXS/SANS) have become popular methods to characterize the structure of membrane proteins, solubilized by either detergents or nanodiscs. SANS studies of protein-detergent complexes usually require deuterium-labeled proteins or detergents, which in turn often lead to problems in their expression or purification. Here, we report an approach whose novelty is the combined analysis of SAXS and SANS data from an unlabeled membrane protein complex in solution in two complementary ways. First, an explicit atomic analysis, including both protein and detergent molecules, using the program WAXSiS, which has been adapted to predict SANS data. Second, the use of MONSA which allows one to discriminate between detergent head- and tail-groups in an ab initio approach. Our approach is readily applicable to any detergent-solubilized protein and provides more detailed structural information on protein–detergent complexes from unlabeled samples than SAXS or SANS alone.

Item Type:Articles
Additional Information:G.D.M. and A.J. were supported by a Ph.D. and a Chancellor’s Fellowship from Strathclyde University, respectively, G.T. and U.Z. acknowledge funding from the Scottish Universities’ Physics Alliance (SUPA). P.A.H. acknowledges the support of the Natural Environment Research Council (NE/M001415/1) and A.J. the support of Tenovus Scotland (Project S17-07). M.T.I., F.M.S., and J.S.H. acknowledge support by the Deutsche Forschungsgemeinschaft (HU 1971-1/1, HU 1971-3/1, HU 1971-4/1).
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Byron, Professor Olwyn
Authors: Dias Mirandela, G., Tamburrino, G., Ivanović, M. T., Strnad, F. M., Byron, O., Rasmussen, T., Hoskisson, P. A., Hub, J. S., Zachariae, U., Gabel, F., and Javelle, A.
College/School:College of Medical Veterinary and Life Sciences > School of Life Sciences
Journal Name:Journal of Physical Chemistry Letters
Publisher:American Chemical Society
ISSN:1948-7185
ISSN (Online):1948-7185
Published Online:25 June 2018
Copyright Holders:Copyright © 2018 American Chemical Society
First Published:First published in Journal of Physical Chemistry Letters 9(14): 3910-3914
Publisher Policy:Reproduced under a Creative Commons License

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