Nelson, C. , Buckley, C., Mullins, J. J., Denvir, M. A. and Taylor, J. (2018) Imaging the Developing Heart: Synchronized Timelapse Microscopy During Developmental Changes. In: SPIE BiOS, San Francisco, CA., 27-28 Jan 2018, (doi: 10.1117/12.2290191)
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Abstract
How do you use imaging to analyse the development of the heart, which not only changes shape but also undergoes constant, high-speed, quasi-periodic changes? We have integrated ideas from prospective and retrospective optical gating to capture long-term, phase-locked developmental time-lapse videos. In this paper we demonstrate the success of this approach over a key developmental time period: heart looping, where large changes in heart shape prevent previous prospective gating approaches from capturing phase- locked videos. We use the comparison with other approaches to in vivo heart imaging to highlight the importance of collecting the most appropriate data for the biological question.
Item Type: | Conference Proceedings |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Nelson, Dr Chas and Taylor, Dr Jonathan |
Authors: | Nelson, C., Buckley, C., Mullins, J. J., Denvir, M. A., and Taylor, J. |
College/School: | College of Science and Engineering > School of Physics and Astronomy |
Copyright Holders: | Copyright © 2018 SPIE |
Publisher Policy: | Reproduced in accordance with the copyright policy of the publisher |
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