Imaging the Developing Heart: Synchronized Timelapse Microscopy During Developmental Changes

Nelson, C. , Buckley, C., Mullins, J. J., Denvir, M. A. and Taylor, J. (2018) Imaging the Developing Heart: Synchronized Timelapse Microscopy During Developmental Changes. In: SPIE BiOS, San Francisco, CA., 27-28 Jan 2018, (doi:10.1117/12.2290191)

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How do you use imaging to analyse the development of the heart, which not only changes shape but also undergoes constant, high-speed, quasi-periodic changes? We have integrated ideas from prospective and retrospective optical gating to capture long-term, phase-locked developmental time-lapse videos. In this paper we demonstrate the success of this approach over a key developmental time period: heart looping, where large changes in heart shape prevent previous prospective gating approaches from capturing phase- locked videos. We use the comparison with other approaches to in vivo heart imaging to highlight the importance of collecting the most appropriate data for the biological question.

Item Type:Conference Proceedings
Glasgow Author(s) Enlighten ID:Nelson, Dr Chas and Taylor, Dr Jonathan
Authors: Nelson, C., Buckley, C., Mullins, J. J., Denvir, M. A., and Taylor, J.
College/School:College of Science and Engineering > School of Physics and Astronomy
Copyright Holders:Copyright © 2018 SPIE
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
642161Development and optimisation of synchronised 3D in-vivo imaging of the embryonic and juvenile zebrafish heartJonathan TaylorBritish Heart Foundation (BHF)NH/14/2/31074S&E P&A - PHYSICS & ASTRONOMY
692111University of Glasgow Experimental Equipment ProposalAndrew HarveyEngineering and Physical Sciences Research Council (EPSRC)EP/M028135/1SCHOOL OF PHYSICS & ASTRONOMY