Hyperglycaemia does not increase perfusion deficits after focal cerebral ischaemia in male Wistar rats

Thow, L. A., MacDonald, K., Holmes, W. M. , Muir, K. W. , Macrae, I. M. and Dewar, D. (2018) Hyperglycaemia does not increase perfusion deficits after focal cerebral ischaemia in male Wistar rats. Brain and Neuroscience Advances, 2, pp. 1-13. (doi: 10.1177/2398212818794820)

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Background: Hyperglycaemia is associated with a worse outcome in acute ischaemic stroke patients; yet the pathophysiological mechanisms of hyperglycaemia-induced damage are poorly understood. We hypothesised that hyperglycaemia at the time of stroke onset exacerbates ischaemic brain damage by increasing the severity of the blood flow deficit. Methods: Adult, male Wistar rats were randomly assigned to receive vehicle or glucose solutions prior to permanent middle cerebral artery occlusion. Cerebral blood flow was assessed semi-quantitatively either 1 h after middle cerebral artery occlusion using 99mTc-D, L-hexamethylpropyleneamine oxime (99mTc-HMPAO) autoradiography or, in a separate study, using quantitative pseudo-continuous arterial spin labelling for 4 h after middle cerebral artery occlusion. Diffusion weighted imaging was performed alongside pseudo-continuous arterial spin labelling and acute lesion volumes calculated from apparent diffusion coefficient maps. Infarct volume was measured at 24 h using rapid acquisition with refocused echoes T2-weighted magnetic resonance imaging. Results: Glucose administration had no effect on the severity of ischaemia when assessed by either 99mTc-HMPAO autoradiography or pseudo-continuous arterial spin labelling perfusion imaging. In comparison to the vehicle group, apparent diffusion coefficient–derived lesion volume 2–4 h post-middle cerebral artery occlusion and infarct volume 24 h post-middle cerebral artery occlusion were significantly greater in the glucose group. Conclusions: Hyperglycaemia increased acute lesion and infarct volumes but there was no evidence that the acute blood flow deficit was exacerbated. The data reinforce the conclusion that the detrimental effects of hyperglycaemia are rapid, and that treatment of post-stroke hyperglycaemia in the acute period is essential but the mechanisms of hyperglycaemia-induced harm remain unclear.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Dewar, Dr Deborah and Macrae, Professor Mhairi and Holmes, Dr William and Muir, Professor Keith and Thow, Dr Lisa
Authors: Thow, L. A., MacDonald, K., Holmes, W. M., Muir, K. W., Macrae, I. M., and Dewar, D.
College/School:College of Medical Veterinary and Life Sciences > School of Psychology & Neuroscience
Journal Name:Brain and Neuroscience Advances
Publisher:SAGE Publications
ISSN (Online):2398-2128
Published Online:20 August 2018
Copyright Holders:Copyright © 2018 The Authors
First Published:First published in Brain and Neuroscience Advances 2: 1-13
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
517244IPF capacity building studentship contributionMargaret MacLeanBritish Pharmacological Society (BRPHASOC)51724/4RI CARDIOVASCULAR & MEDICAL SCIENCES